Proteomic and Functional Genomic Landscape of Receptor Tyrosine Kinase and Ras to Extracellular Signal–Regulated Kinase Signaling

Adam A. Friedman, George Tucker, Rohit Singh, Dong Yan, Arunachalam Vinayagam, Yanhui Hu, Richard Binari, Pengyu Hong, Xiaoyun Sun, Maura Porto, Svetlana Pacifico, Thilakam Murali, Russell L. Finley Jr., John M. Asara, Bonnie Berger, Norbert Perrimon*

*To whom correspondence should be addressed. E-mail: perrimon{at}receptor.med.harvard.edu

This PDF file includes:

• Fig. S1. Primary RNAi screen optimization and data.
• Fig. S2. Performance of SAINT-based PPI ranking.
• Fig. S3. Validation of additional PPI interactions.
• Fig. S4. Phenotypes of additional RTK-Ras-ERK network components based on in vivo RNAi.
• Table S3. RTK-specific hit subsets.
  

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Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

• Table S1. Compilation of all hits from six genome-wide RNAi screens performed in duplicate, and grouping by screen type.
• Table S2. Gene Ontology Biological Process and Molecular Function categories enriched in RNAi screen hits and PPI data.
• Table S4. Protein-protein interactions determined by TAP-MS analysis of RTK-Ras-ERK canonical pathway.
• Table S5. Compilation of all in vivo results.
• Table S6. Hairpin lines used for in vivo analyses.
• Cytoscape file for the PPI and RNAi data.
• Excel spreadsheets of peptides isolated in each TAP-MS experiment.

Technical Details

Format: Microsoft Excel

Size: 2.5 MB (compressed); 8.11 MB (decompressed)

Technical Details

Format: Cytoscape

Size: 41 KB (compressed); 42.3 MB (decompressed)

Technical Details

Format: Microsoft Excel

Size: 4.5 MB (compressed); 13.2 MB (decompressed)

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© 2011 American Association for the Advancement of Science