Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 8 November 2011
[DOI: 10.1126/scisignal.2001614]

Supplementary Materials for:

Short RNA Duplexes Elicit RIG-I–Mediated Apoptosis in a Cell Type– and Length-Dependent Manner

Osamu Ishibashi, Md. Moksed Ali, Shan-Shun Luo, Takashi Ohba, Hidetaka Katabuchi, Toshiyuki Takeshita, Toshihiro Takizawa*

*To whom correspondence should be addressed. E-mail: t-takizawa{at}

This PDF file includes:

  • Fig. S1. Pre-351 reduced the viability of KGN cells regardless of the transfection method used.
  • Fig. S2. Sequences of miRs-125b, -351, and -125a-5p.
  • Fig. S3. Dose-dependent effect of poly(I:C) on KGN cell viability.
  • Fig. S4. Effects of pre-miRs on HL-60 cell viability.
  • Fig. S5. Pre-351–induced apoptosis of GC1 cells.
  • Fig. S6. Subcellular translocation of cytochrome c and NF-κB in KGN cells transfected with Pre-351.
  • Fig. S7. Pre-351 reduced the viability of KGN cells in an IFN-independent manner.
  • Fig. S8. MDA5 is not involved in Pre-351–induced apoptosis of KGN cells.
  • Fig. S9. siRNA-mediated knockdown of RIG-I in KGN cells.
  • Fig. S10. The transcriptional induction of a representative ISG (MX1) by Pre-351 was mostly unaffected by knockdown of RIG-I.
  • Fig. S11. Pretreatment of HeLa cells with IFN-β potentiated the ability of Pre-351 to enhance caspase-3 and caspase-7 activities.
  • Fig. S12. Knockdown of RIG-I in HuCCT1 cells by siRNA.
  • Fig. S13. Pre-351 induced the phosphorylation of p38 in KGN cells.
  • Fig. S14. PKRI- and RIG-I–dependent Pre-351–induced phosphorylation of p38.
  • Fig. S15. Basal abundance of RIG-I mRNA in KGN and HeLa cells.
  • Fig. S16. Schematic model of short dsRNA–induced apoptotic signaling mediated by PKR, RIG-I, and p38 MAPK.
  • Table S1. Genes whose expression was increased (>50-fold) in KGN cells after transfection with Pre-351 as compared to their expression in cells transfected with Pre-Cont.
  • Table S2. List of siRNAs used in this study.
  • Table S3. Primers used for real-time PCR analysis.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.57 MB

Citation: O. Ishibashi, M. M. Ali, S.-S. Luo, T. Ohba, H. Katabuchi, T. Takeshita, T. Takizawa, Short RNA Duplexes Elicit RIG-I–Mediated Apoptosis in a Cell Type– and Length-Dependent Manner. Sci. Signal. 4, ra74 (2011).

© 2011 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882