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Sci. Signal., 8 November 2011
[DOI: 10.1126/scisignal.2001614]

Supplementary Materials for:

Short RNA Duplexes Elicit RIG-I–Mediated Apoptosis in a Cell Type– and Length-Dependent Manner

Osamu Ishibashi, Md. Moksed Ali, Shan-Shun Luo, Takashi Ohba, Hidetaka Katabuchi, Toshiyuki Takeshita, Toshihiro Takizawa*

*To whom correspondence should be addressed. E-mail: t-takizawa{at}nms.ac.jp

This PDF file includes:

  • Fig. S1. Pre-351 reduced the viability of KGN cells regardless of the transfection method used.
  • Fig. S2. Sequences of miRs-125b, -351, and -125a-5p.
  • Fig. S3. Dose-dependent effect of poly(I:C) on KGN cell viability.
  • Fig. S4. Effects of pre-miRs on HL-60 cell viability.
  • Fig. S5. Pre-351–induced apoptosis of GC1 cells.
  • Fig. S6. Subcellular translocation of cytochrome c and NF-κB in KGN cells transfected with Pre-351.
  • Fig. S7. Pre-351 reduced the viability of KGN cells in an IFN-independent manner.
  • Fig. S8. MDA5 is not involved in Pre-351–induced apoptosis of KGN cells.
  • Fig. S9. siRNA-mediated knockdown of RIG-I in KGN cells.
  • Fig. S10. The transcriptional induction of a representative ISG (MX1) by Pre-351 was mostly unaffected by knockdown of RIG-I.
  • Fig. S11. Pretreatment of HeLa cells with IFN-β potentiated the ability of Pre-351 to enhance caspase-3 and caspase-7 activities.
  • Fig. S12. Knockdown of RIG-I in HuCCT1 cells by siRNA.
  • Fig. S13. Pre-351 induced the phosphorylation of p38 in KGN cells.
  • Fig. S14. PKRI- and RIG-I–dependent Pre-351–induced phosphorylation of p38.
  • Fig. S15. Basal abundance of RIG-I mRNA in KGN and HeLa cells.
  • Fig. S16. Schematic model of short dsRNA–induced apoptotic signaling mediated by PKR, RIG-I, and p38 MAPK.
  • Table S1. Genes whose expression was increased (>50-fold) in KGN cells after transfection with Pre-351 as compared to their expression in cells transfected with Pre-Cont.
  • Table S2. List of siRNAs used in this study.
  • Table S3. Primers used for real-time PCR analysis.

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Citation: O. Ishibashi, M. M. Ali, S.-S. Luo, T. Ohba, H. Katabuchi, T. Takeshita, T. Takizawa, Short RNA Duplexes Elicit RIG-I–Mediated Apoptosis in a Cell Type– and Length-Dependent Manner. Sci. Signal. 4, ra74 (2011).

© 2011 American Association for the Advancement of Science


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