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Sci. Signal., 15 November 2011
[DOI: 10.1126/scisignal.2002011]

Supplementary Materials for:

Local Application of Neurotrophins Specifies Axons Through Inositol 1,4,5-Trisphosphate, Calcium, and Ca2+/Calmodulin–Dependent Protein Kinases

Shinichi Nakamuta, Yasuhiro Funahashi, Takashi Namba, Nariko Arimura, Marina R. Picciotto, Hiroshi Tokumitsu, Thomas R. Soderling, Akira Sakakibara, Takaki Miyata, Hiroyuki Kamiguchi, Kozo Kaibuchi*

*To whom correspondence should be addressed. E-mail: kaibuchi{at}med.nagoya-u.ac.jp

This PDF file includes:

  • Materials and Methods
  • Fig. S1. TrkB and TrkC signaling contributes to neuronal polarization.
  • Fig. S2. NT-3–stimulated neurite becomes an axon.
  • Fig. S3. Local application of BDNF induces neurite outgrowth.
  • Fig. S4. CaMKK and CaMKI inhibitors prevent neuronal polarization.
  • Fig. S5. CaMKKα-CA induces multiple axons.
  • Fig. S6. CaMKIα contributes to neuronal polarization.
  • Fig. S7. CaMKKα is expressed in multipolar neurons.
  • Fig. S8. Migration and morphology of cortical neurons.
  • Fig. S9. Morphological analysis of EGFP+ multipolar cells.
  • Fig. S10. CaMKKα contributes to neuronal polarization in vivo.
  • Fig. S11. Expression of CaMKKα-DN under the control of the CAG promoter impairs neuronal migration in vivo.
  • Fig. S12. Expression of TrkB, TrkC, or CaMKKα impairs neuronal polarization and neuronal migration in vivo at E18.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 3 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Multipolar-to-bipolar transition of neurons in the embryonic neocortex.
  • Movie S2 (.mp4 format). Migration and morphology of control cortical neurons.
  • Movie S3 (.mp4 format). Migration and morphology of cortical neurons electroporated with wild-type CaMKKa.
  • Movie S4 (.mp4 format). Migration and morphology of cortical neurons electroporated with CaMKKα-CA.
  • Movie S5 (.mp4 format). Migration and morphology of cortical neurons electroporated with CaMKKα-DN.

Citation: S. Nakamuta, Y. Funahashi, T. Namba, N. Arimura, M. R. Picciotto, H. Tokumitsu, T. R. Soderling, A. Sakakibara, T. Miyata, H. Kamiguchi, K. Kaibuchi, Local Application of Neurotrophins Specifies Axons Through Inositol 1,4,5-Trisphosphate, Calcium, and Ca2+/Calmodulin–Dependent Protein Kinases. Sci. Signal. 4, ra76 (2011).

© 2011 American Association for the Advancement of Science


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