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Sci. Signal., 22 November 2011
[DOI: 10.1126/scisignal.2002208]

Supplementary Materials for:

Agonist-Driven Maturation and Plasma Membrane Insertion of Calcium-Sensing Receptors Dynamically Control Signal Amplitude

Michael P. Grant, Ann Stepanchick, Alice Cavanaugh, Gerda E. Breitwieser*

*To whom correspondence should be addressed. E-mail: gebreitwieser{at}geisinger.edu

This PDF file includes:

  • Fig. S1. Agonist-driven insertion of CaSRs but not AT1Rs.
  • Fig. S2. CaSRs do not recycle to the PM after stimulation but are targeted to the lysosome for degradation.
  • Fig. S3. ADIS requires signaling by PM CaSR and release from secretory compartments.
  • Fig. S4. Endogenous CaSRs in HUVECs undergo ADIS.
  • Fig. S5. CaSR interactions with 14-3-3 proteins are modulated by C-terminal mutations and encompass different 14-3-3 subtypes.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 543 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). TIRFM of BTx-A594–labeled BSEP-CaSR stimulated with extracellular Ca2+.

Technical Details

Format: AVI

Size: 1.27 MB


Citation: M. P. Grant, A. Stepanchick, A. Cavanaugh, G. E. Breitwieser, Agonist-Driven Maturation and Plasma Membrane Insertion of Calcium-Sensing Receptors Dynamically Control Signal Amplitude. Sci. Signal. 4, ra78 (2011).

© 2011 American Association for the Advancement of Science


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