Ric-8 Proteins Are Molecular Chaperones That Direct Nascent G Protein α Subunit Membrane Association

Meital Gabay, Mary E. Pinter, Forrest A. Wright, PuiYee Chan, Andrew J. Murphy, David M. Valenzuela, George D. Yancopoulos, Gregory G. Tall*

*To whom correspondence should be addressed. E-mail: gregory_tall{at}urmc.rochester.edu

This PDF file includes:

• Fig. S1. Genomic PCR scheme to genotype Ric-8A– and Ric-8B–deleted mice and derived mES cell lines.
• Fig. S2. Increased abundance of Gβγ does not rescue the defects in Gα protein abundance observed in Ric-8^–/– cells.
• Fig. S3. Nascent Gα_i appears to be differentially modified in Ric-8A^–/– and wild-type cells by means other than myristoylation.
• Fig. S4. Forskolin-stimulated cAMP production is blunted in Ric-8A^–/– and Ric-8B^–/– cells compared to that in wild-type cells.

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© 2011 American Association for the Advancement of Science