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Sci. Signal., 29 November 2011
[DOI: 10.1126/scisignal.2002189]

Supplementary Materials for:

SH3 Domain–Based Phototrapping in Living Cells Reveals Rho Family GAP Signaling Complexes

Hirokazu Okada, Akiyoshi Uezu, Frank M. Mason, Erik J. Soderblom, M. Arthur Moseley III, Scott H. Soderling*

*To whom correspondence should be addressed. E-mail: s.soderling{at}cellbio.duke.edu

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Expression levels of Rho family GAP SH3 domains and their interactors.
  • Fig. S2. Identification of SH3 domain binding proteins by peptide array–based in vitro binding assay and coimmunoprecipitation.
  • Fig. S3. Rho family GAP PI cluster interactomes and colocalization data.
  • Fig. S4. Gephyrin's interaction with WRP and colocalization with VIAAT.
  • Fig. S5. Endogenous gephyrin is highly colocalized with inhibitory synapse presynaptic marker VIAAT in vivo.
  • Reference

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Technical Details

Format: Adobe Acrobat PDF

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Citation: H. Okada, A. Uezu, F.M.Mason, E. J. Soderblom,M. A.Moseley III, S. H. Soderling, SH3 domain–based phototrapping in living cells reveals Rho family GAP signaling complexes. Sci. Signal. 4, rs13 (2011).

© 2011 American Association for the Advancement of Science


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