The SH2 Domain–Containing Proteins in 21 Species Establish the Provenance and Scope of Phosphotyrosine Signaling in Eukaryotes

Bernard A. Liu, Eshana Shah, Karl Jablonowski, Andrew Stergachis, Brett Engelmann, Piers D. Nash*

*To whom correspondence should be addressed. E-mail: pdnash.uchicago{at}gmail.com

This PDF file includes:

• Section S1. SH2 domain proteins in organisms with incomplete genomes.
• Section S2. Analysis of the intron/exon code of human SH2 domains.
• Section S3. Comparative analysis of ortholog and paralog predictions.
• Fig. S1. An evolutionary time line for the organisms represented in this study.
• Fig. S2. Evolutionary expansion of SH2 domains, tyrosine kinases, and PDZ domains.
• Fig. S3. Splice site positions within the protein sequence alignment of human SH2 domains.
• Fig. S4. The bead on a string representation for protein domains.
• Fig. S5. Gene duplication and loss within SH2 domain families.
• Fig. S6. Clustal alignments of the GRB2 and CRK families.
• Fig. S7. Insertion of an intramolecular phosphorylation site for autoinhibition of CRK.
• Fig. S8. Evolving new SH2 interactions through novel pTyr sites.
• Fig. S9. Conservation of the pTyr ligand-binding pocket in SH2 domains.
• Fig. S10. Tissue expression of human SH2 domain proteins.
• Table S1. A complete list of organisms in this study.
• Table S2. SH2 domains in Bikonta and Amoebozoa.
• Table S5. Classification of SH2 domain family divergence.
• Table S6. Defining SH2 families with Ensembl paralog predictions versus family organization.
• Table S7. Ortholog predictions of human SH2 proteins to proteins in lower organisms.
• Table S8. Ortholog predictions from C. elegans to H. sapiens.
• References
  

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Other Supplementary Material for this manuscript includes the following:

• Table S3. Comprehensive list of SH2 domain–containing proteins in Eukaryotes (Excel file).
• Table S4. Comprehensive list of SH2 domain–containing proteins in organisms with incomplete genome annotations (Excel file).

[Download Tables S3. and S4. (compressed)]

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© 2011 American Association for the Advancement of Science