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Sci. Signal., 6 December 2011
[DOI: 10.1126/scisignal.2002058]

Supplementary Materials for:

Protein Kinase C η Is Required for T Cell Activation and Homeostatic Proliferation

Guo Fu,* Jianfang Hu, Nathalie Niederberger-Magnenat, Vasily Rybakin, Javier Casas, Pia P. Yachi, Stephanie Feldstein, Bo Ma, John A. H. Hoerter, Jeanette Ampudia, Stephanie Rigaud, Florence Lambolez, Amanda L. Gavin, Karsten Sauer, Hilde Cheroutre, Nicholas R. J. Gascoigne*

*To whom correspondence should be addressed. E-mail: gascoigne{at}scripps.edu (N.R.J.G.); guofu{at}scripps.edu (G.F.)

This PDF file includes:

  • Fig. S1. Recruitment of PKCη to the immunological synapse in the presence of PKCθ.
  • Fig. S2. Recruitment of PKCη to the immunological synapse in preselection thymocytes in the absence of PKCθ.
  • Fig. S3. Antigen-specific recruitment of PKCη to the immunological synapse.
  • Fig. S4. Time course of the recruitment of PKCη and PKCθ to the immunological synapse.
  • Fig. S5. Generation of PKCη–/– mice.
  • Fig. S6. Analysis of CD4+ T cell subsets.
  • Fig. S7. Cell death status of wild-type and PKCη KO cells responding to antigen in vivo.
  • Fig. S8. TCR signaling cascade in mature T cells from PKCη–/– mice.
  • Fig. S9. Comparison of peripheral T cell phenotypes in three PKC-deficient mouse strains.
  • Fig. S10. IL-7Rα (CD127) and IL-15R (CD122) abundances on PKC-deficient cells are normal.
  • References

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Citation: G. Fu, J. Hu, N. Niederberger-Magnenat, V. Rybakin, J. Casas, P. P. Yachi, S. Feldstein, B. Ma, J. A. H. Hoerter, J. Ampudia, S. Rigaud, F. Lambolez, A. L. Gavin, K. Sauer, H. Cheroutre, N. R. J. Gascoigne, Protein Kinase C η Is Required for T Cell Activation and Homeostatic Proliferation. Sci. Signal. 4, ra84 (2011).

© 2011 American Association for the Advancement of Science


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