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Sci. Signal., 13 December 2011
[DOI: 10.1126/scisignal.2002033]

Supplementary Materials for:

Triggering Actin Comets Versus Membrane Ruffles: Distinctive Effects of Phosphoinositides on Actin Reorganization

Tasuku Ueno, Björn H. Falkenburger, Christopher Pohlmeyer, Takanari Inoue*

*To whom correspondence should be addressed. E-mail: jctinoue{at}jhmi.edu

This PDF file includes:

  • Fig. S1. Kinetics of PH(PLCδ) dynamics upon PDGF-induced PI(4,5)P2 depletion.
  • Fig. S2. Quantitative analysis of PI(4,5)P2 and model for phosphatidylinositol
  • dynamics upon PI(4,5)P2 manipulation.
  • Fig. S3. Actin phenotype upon recruitment of FKBP-tagged PI 5-kinase.
  • Fig. S4. Actin phenotype upon FKBP-tagged lipid binder sequestration.
  • Fig. S5. Confocal fluorescence microscopy images of cells after phalloidin staining upon PI(4,5)P2 manipulation.
  • Fig. S6. Actin phenotypes in HeLa and HEK293 cells upon PI(4,5)P2 synthesis and liberation.
  • Fig. S7. Evaluation of endogenous PI(4)P5K activity by means of KCNQ2/3 current in the presence of ROCK inhibitor.
  • Fig. S8. Actin phenotypes and actin-binding molecules.
  • Fig. S9. PI(4)P depletion does not induce actin phenotype.
  • Fig. S10. Synthetic reconstitution of actin comets.
  • Table S1. Initial conditions used for the model.
  • Table S2. Differential equations used for the model.
  • Table S3. Summary of statistical analyses, including P values.

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Size: 2.32 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.AVI and .mov format). Inducible recruitment of PI(4)P5K to plasma membrane.

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Formats: QuickTime and Windows Media Player movies

Size: 1.2 MB / 12.2 MB

  • Movie S2 (.AVI and .mov). Inducible sequestration of PH(PLCδ) from plasma membrane.

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Size: 1 MB / 17.9 MB

  • Movie S3 (.AVI and .mov). PI(4,5)P2 synthesis induces actin comets.

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Formats: QuickTime and Windows Media Player movies

Size: 479 KB / 28.3 MB

  • Movie S4 (.AVI and .mov). PI(4,5)P2 liberation induces membrane ruffles.

Technical Details

Formats: QuickTime and Windows Media Player movies

Size: 978 KB / 28.3 MB

 


Citation: T. Ueno, B. H. Falkenburger, C. Pohlmeyer, T. Inoue, Triggering Actin Comets Versus Membrane Ruffles: Distinctive Effects of Phosphoinositides on Actin Reorganization. Sci. Signal. 4, ra87 (2011).

© 2011 American Association for the Advancement of Science


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