Human Regulatory T Cells Rapidly Suppress T Cell Receptor–Induced Ca²⁺, NF-κB, and NFAT Signaling in Conventional T Cells

Angelika Schmidt, Nina Oberle, Eva-Maria Weiß, Diana Vobis, Stefan Frischbutter, Ria Baumgrass, Christine S. Falk, Mathias Haag, Britta Brügger, Hongying Lin, Georg W. Mayr, Peter Reichardt, Matthias Gunzer, Elisabeth Suri-Payer, Peter H. Krammer*

*To whom correspondence should be addressed. E-mail: P.Krammer{at}dkfz.de

This PDF file includes:

• Methods
• Fig. S1. Suppression of cytokine secretion is retained after removal of T_regs.
• Fig. S2. T_reg-mediated rapid suppression of IL2 expression in T_cons also occurs in the presence of APCs.
• Fig. S3. CTLA-4 is not involved in the suppression of cytokine gene expression.
• Fig. S4. Proximal TCR signaling is not altered in suppressed T_cons.
• Fig. S5. Suppression of NFAT and NF-κB activation is independent of the antibody used to activate T_regs.
• Fig. S6. Phosphorylation of ERK and p38 is not affected in suppressed T_cons.
• Fig. S7. The block in Ca²⁺ influx in T_cons is not caused by allogeneic responses and requires 30 min of previous coculture with T_regs.
• Fig. S8. Suppression of Ca²⁺ signaling is independent of cAMP.
• Fig. S9. T_regs, but not T_cons, pre-activated with covalently plate-bound antibody against CD3 suppress Ca²⁺ signaling in responder T_cons.
• Fig. S10. NFAT and NF-κB activation in T_cons is Ca²⁺-dependent.
• References
  

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© 2011 American Association for the Advancement of Science