Incoherent Feedforward Control Governs Adaptation of Activated Ras in a Eukaryotic Chemotaxis Pathway

Kosuke Takeda, Danying Shao, Micha Adler, Pascale G. Charest, William F. Loomis, Herbert Levine, Alex Groisman, Wouter-Jan Rappel,* Richard A. Firtel*

*To whom correspondence should be addressed. E-mail: rappel{at}physics.ucsd.edu (W.-J.R.); rafirtel{at}ucsd.edu (R.A.F.)

This PDF file includes:

• Text S1. Effects of two species of receptors.
• Text S2. Model equations.
• Text S3. Data fitting.
• Text S4. Analytical analysis of the incoherent feedforward and the integral control topologies.
• Text S5. Spatially extended models.
• Fig. S1. Detailed drawing of the microfluidic device.
• Fig. S2. Membrane-associated RBD-GFP is inversely related to cytosolic RBD-GFP fluorescence.
• Fig. S3. Responses in latrunculin-treated cells.
• Fig. S4. Effects of different receptor populations.
• Fig. S5. Response times for the different models.
• Fig. S6. Possible integral control topologies.
• Fig. S7. Possible incoherent feedforward topologies.
• Fig. S8. Core model topologies.
• Fig. S9. Comparison of the response in a spatially uniform and spatially extended model.
• Table S1. Model parameters used in this study.
• References
  

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