MNK2 Inhibits eIF4G Activation Through a Pathway Involving Serine-Arginine–Rich Protein Kinase in Skeletal Muscle

Shou-Ih Hu, Mark Katz, Sherry Chin, Xiaoqing Qi, Joseph Cruz, Chikwendu Ibebunjo, Shanchuan Zhao, Amy Chen, David J. Glass*

*To whom correspondence should be addressed. E-mail: david.glass{at}novartis.com

This PDF file includes:

• Fig. S1. Densitometry quantification of the ratio of phospho/total eIF4G and eIF4E from Fig. 1A.
• Fig. S2. Densitometry quantification of the ratio of phospho/total eIF4G and p70S6K from Fig. 3A.
• Fig. S3. MNK2 overexpression partially inhibits global protein synthesis.
• Fig. S4. MNK2 and Pras40 compete for binding to Raptor.
• Fig. S5. MNK2 and SRPK1 interact with eIF4G.
• Fig. S6. Densitometry quantification of the ratio of phospho/total eIF4G in Fig. 5.
• Fig. S7. Expression of atrophy-associated genes and eIF4G phosphorylation status in gastrocnemius of a denervation-induced atrophy model.
• Fig. S8. Muscle mass in dexamethasone-induced and denervation-induced atrophy models.
• Fig. S9. Proposed negative role of MNK2 on protein translation through SRPK and TORC1.
• Table S1 legend
  

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 367 KB

Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). Summary of primary mouse kinase panel siRNA screen.

[Download Table S1]

Technical Details

Format:  Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 24 KB (compressed); 56 KB (decompressed)

Instructions for downloading and decompressing files:

1. Create a temporary folder on your machine's hard drive.
2. Save the compressed archive to the temporary folder you created, using the links above.
3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; www.winzip.com) or StuffIt Expander (Windows and Mac; www.stuffit.com).

© 2012 American Association for the Advancement of Science