Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 21 February 2012
[DOI: 10.1126/scisignal.2002202]

Supplementary Materials for:

RGS Proteins Maintain Robustness of GPCR-GIRK Coupling by Selective Stimulation of the G Protein Subunit Gαo

Huai-hu Chuang* and Alexander Y. Chuang

*To whom correspondence should be addressed. E-mail: huai-hu.chuang{at}cornell.edu

This PDF file includes:

  • Materials and Methods
  • Mathematical simulation of G protein activation for Fig. 1A
  • Membrane-targeted RGS constructs
  • Fig. S1. Deactivation kinetics of A1 or m2 for various RGS proteins.
  • Fig. S2. RGS4 does not stimulate β2-AR–stimulated Gαs-dependent GIRK currents.
  • Fig. S3. Deactivation kinetics of membrane-associated RGS box constructs.
  • Fig. S4. Deactivation kinetics and ACh dose-response curves of oocytes with or without the full-length RGS4N128A.
  • Fig. S5. Deactivation kinetics of the lyn-tagged R4Box and mutants.
  • Fig. S6. Effects of PTX treatment.
  • Fig. S7. RGS expression level–dependent deactivation kinetics.
  • Fig. S8. Increased RGS8 expression enhanced the acceleration of deactivation kinetics.
  • Fig. S9. GTP-γ-S induced GIRK activation in Gαi1-expressing oocyte membranes.
  • Fig. S10. RGS4 exhibited its GAP effect on Gαi1 R178M.
  • Table S1. P values for comparison of RGS effects on current enhancement in Fig. 1D.
  • Table S2. P values for stimulatory effects on GIRK current by various membrane-targeted RGS box constructs.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 212 KB


Citation: H.-h. Chuang, A. Y. Chuang, RGS Proteins Maintain Robustness of GPCR-GIRK Coupling by Selective Stimulation of the G Protein Subunit Gαo. Sci. Signal. 5, ra15 (2012).

© 2012 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882