Diverse Sensitivity Thresholds in Dynamic Signaling Responses by Social Amoebae

C. Joanne Wang, Adriel Bergmann, Benjamin Lin, Kyuri Kim, Andre Levchenko*

*To whom correspondence should be addressed. E-mail: alev{at}jhu.edu

This PDF file includes:

• Text S1. Determining the threshold for partitioning responders from nonresponders.
• Text S2. Comparing the responses of single integral feedback and feedforward loops to ramp input.
• Text S3. Method for gradient sensing analysis.
• Text S4. Setup and development of the collective cell response model.
• Text S5. Mathematical description of the aLEGI model.
• Fig. S1. The microfluidic function generator.
• Fig. S2. The properties of aLEGI model.
• Fig. S3. Schematic mechanism of increased rate of cAMP wave propagation as a function of the colony heterogeneity.
• Fig. S4. Simulation of the average dose-response dynamics.
• Fig. S5. Dependence of PIP₃ responses on probe expression.
• Fig. S6. Distribution of cell responses to step stimulation at two different developmental stages.
• Fig. S7. Responses after latrunculin A treatment.
• References
• Movies S1 to S14 legends
  

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.17 MB

Other Supplementary Material for this manuscript includes the following:

• Movies S1 to S14 (.avi format). Translocation kinetics of PHcrac-GFP in cells stimulated under various conditions.

[Download Movies S1 to S14 (Compressed)]

Technical Details

Format: Windows Media Player movies

Size: 31.62 MB (compressed)

Instructions for downloading and decompressing files:

1. Create a temporary folder on your machine's hard drive.
2. Save the compressed archive to the temporary folder you created, using the links above.
3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; www.winzip.com) or StuffIt Expander (Windows and Mac; www.stuffit.com).

© 2012 American Association for the Advancement of Science