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Sci. Signal., 28 February 2012
[DOI: 10.1126/scisignal.2002138]

Supplementary Materials for:

PTEN Protein Phosphatase Activity Correlates with Control of Gene Expression and Invasion, a Tumor-Suppressing Phenotype, But Not with AKT Activity

Priyanka Tibarewal, Georgios Zilidis, Laura Spinelli, Nick Schurch, Helene Maccario, Alexander Gray, Nevin M. Perera, Lindsay Davidson, Geoffrey J. Barton, Nick R. Leslie*

*To whom correspondence should be addressed. E-mail: n.r.leslie{at}dundee.ac.uk

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Regulation of AKT phosphorylation by wild-type PTEN and PTEN Y138L.
  • Fig. S2. Assigned gene sets associated with transcript changes induced by wild-type PTEN expression in U87MG cells.
  • Fig. S3. Autodephosphorylation analysis of PTEN mutant proteins.
  • Fig. S4. Effects of kinase inhibition and hyperosmotic stress on PTEN Thr366 phosphorylation.
  • Fig. S5. Further analysis of PTEN Thr366 phosphorylation.
  • Fig. S6. Effects of PTEN on U87MG cell morphology in 3D Matrigel cultures.
  • Fig. S7. Quantitation of blotting data.
  • Fig. S8. Quantitation and statistical analysis of blotting data.
  • Table S1. Wild-type PTEN–responsive gene list used for clinical data comparison.
  • References

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Technical Details

Format: Adobe Acrobat PDF

Size: 2.45 MB


Citation: P. Tibarewal, G. Zilidis, L. Spinelli, N. Schurch, H. Maccario, A. Gray, N. M. Perera, L. Davidson, G. J. Barton, N. R. Leslie, PTEN Protein Phosphatase Activity Correlates with Control of Gene Expression and Invasion, a Tumor-Suppressing Phenotype, But Not with AKT Activity. Sci. Signal. 5, ra18 (2012).

© 2012 American Association for the Advancement of Science


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