Regulation of the EGF Transcriptional Response by Endocytic Sorting

Ben Brankatschk, Sven P. Wichert, Shem D. Johnson, Olivier Schaad, Moritz J. Rossner, Jean Gruenberg*

*To whom correspondence should be addressed. E-mail: jean.gruenberg{at}unige.ch

This PDF file includes:

• Text S1. Analysis of the different conditions of stimulation.
• Text S2. Quality control for the second NanoString analysis.
• Text S3. Quality control for the third NanoString analysis.
• Fig. S1. Quantification of EGFR, as well as phosphorylated EGFR and downstream kinases, after depletion of ESCRT proteins.
• Fig. S2. Quality controls for microarrays and the first NanoString measurement.
• Fig. S3. EGF-induced feedback regulators identified in the microarray analysis.
• Fig. S4. Magnitude and comparison of ESCRT knockdown effects in the microarrays, and network analysis of HRS and TSG101 knockdown effects.
• Fig. S5. Quantification of EGFR, as well as phosphorylated EGFR and downstream kinases, under different conditions of stimulation with EGF.
• Fig. S6. Quality controls for the second NanoString experiment and comparison to the first data set.
• Fig. S7. Quantification of abundance and phosphorylation of EGFR and phosphorylation of downstream kinases after various double knockdowns.
• Fig. S8. Analysis of EGFR distribution.
• Fig. S9. EGFR localization after 60 min of EGF stimulation.
• Fig. S10. Hierarchical clustering of the transcriptional response and of effects in the second NanoString experiment.
• Fig. S11. Hierarchical clustering of effects in the third NanoString experiment and quality controls.
• Table S1. Two-way ANOVA analysis of quantification of EGFR and phosphorylated EGFR, MEK1/2, or ERK1/2.
• Table S2. Microarray analysis of EGF response genes.
• Table S3. Microarray analysis of knockdown effects.
• Table S4. Target sequences of the NanoString code sets.
• Table S5. siRNA target sequences.
• Table S6. Microarray analysis of all EGF response genes.
• References
  

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© 2012 American Association for the Advancement of Science