Astrocytes Modulate Neural Network Activity by Ca²⁺-Dependent
Uptake of Extracellular K⁺

Fushun Wang, Nathan A. Smith, Qiwu Xu, Takumi Fujita, Akemichi Baba, Toshio Matsuda, Takahiro Takano, Lane Bekar, Maiken Nedergaard*

*To whom correspondence should be addressed. E-mail: nedergaard{at}urmc.rochester.edu

This PDF file includes:

• Fig. S1. Ouabain-insensitive ⁸⁶Rb⁺ uptake.
• Fig. S2. Doxycycline suppresses eGFP expression in cultured astrocytes prepared from MrgA1^+/− mice.
• Fig. S3. GPCR-activated increases in ⁸⁶Rb⁺ uptake require increases in cytosolic Ca²⁺ in cultured astrocytes.
• Fig. S4. Comparison of ⁸⁶Rb⁺ uptake in rat versus mouse and rat cortical versus hippocampal astrocytes.
• Fig. S5. Detection of GPCR-activated changes in EPSPs is depth- and [K⁺]_Bath-dependent.
• Fig. S6. GPCR activation triggers a transient minor increase in intra-astrocytic Na⁺ concentration and a minor decrease in extracellular Ca²⁺ concentration in hippocampal slices.
• Fig. S7. Activation of GPCR-induced changes in PKA and PKC activity does not mediate ATP-induced increases in ⁸⁶Rb⁺ uptake in cultured astrocytes.
• Fig. S8. Combined recordings of extracellular K⁺ with mEPSCs or mIPSCs in hippocampal slices.
• Fig. S9. Effects of GPCR activation or reduction of bath K⁺ concentration on extracellular K⁺, neuronal membrane potential, and evoked EPSP in hippocampal slices.
  

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© 2012 American Association for the Advancement of Science