Perinuclear Mitochondrial Clustering Creates an Oxidant-Rich Nuclear Domain Required for Hypoxia-Induced Transcription

Abu-Bakr Al-Mehdi, Viktor M. Pastukh, Brad M. Swiger, Darla J. Reed, Mita R. Patel, Gina C. Bardwell, Viktoriya V. Pastukh, Mikhail F. Alexeyev, Mark N. Gillespie*

*To whom correspondence should be addressed. E-mail: mgillesp{at}southalabama.edu

This PDF file includes:

• Fig. S1. Effect of hypoxia, microtubule inhibition, and dynein motor suppression on microtubule morphology and mitochondrial distribution.
• Fig. S2. Involvement of ROS in hypoxia-induced perinuclear mitochondrial clustering.
• Fig. S3. Distribution and dynamic range of nontargeted roGFP.
• Fig. S4. Effect of perinuclear mitochondrial clustering on DCF-detectable hypoxia-induced ROS production.
• Fig. S5. Targeting of roGFP to the nucleus.
• Fig. S6. HREs of the NOS2 and NFKB2 promoters show hypoxia-inducible oxidative base modifications that are attenuated by preventing perinuclear clustering of mitochondria.
  

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© 2012 American Association for the Advancement of Science