Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 14 August 2012
[DOI: 10.1126/scisignal.2002720]

Supplementary Materials for:

Sequential Inter- and Intrasubunit Rearrangements During Activation of Dimeric Metabotropic Glutamate Receptor 1

Veronika Hlavackova, Ulrike Zabel, Daniela Frankova, Julia Bätz, Carsten Hoffmann, Laurent Prezeau, Jean-Philippe Pin,* Jaroslav Blahos,* Martin J. Lohse*

*To whom correspondence should be addressed. E-mail: lohse{at}toxi.uni-wuerzburg.de (M.J.L.); blahos{at}img.cas.cz (J.B.); Jean-Philippe.Pin{at}igf.cnrs.fr (J.-P.P.)

This PDF file includes:

  • Fig. S1. mGluR1 constructs for intersubunit FRET are present on the cell surface.
  • Fig. S2. Uncoupling of the VFT domain of the A-sensor subunit abolishes agonist-induced changes in FRET.
  • Fig. S3. Formation of heterodimers with the A-sensor.
  • Table S1. Positioning of the fluorophores within the mGlu1-based receptor FRET sensors.
  • Table S2. Values for the Hill coefficient (h) and 95% confidence interval for concentration-response curves.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.2 MB

Citation: V. Hlavackova, U. Zabel, D. Frankova, J. Bätz, C. Hoffmann, L. Prezeau, J.-P. Pin, J. Blahos, M. J. Lohse, Sequential Inter- and Intrasubunit Rearrangements During Activation of Dimeric Metabotropic Glutamate Receptor 1. Sci. Signal. 5, ra59 (2012).

© 2012 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882