Network Analysis of the Focal Adhesion to Invadopodia Transition Identifies a PI3K-PKCα Invasive Signaling Axis

Daisuke Hoshino, Jerome Jourquin, Shane Weller Emmons, Tyne Miller, Margalit Goldgof, Kaitlin Costello, Darren R. Tyson, Brandee Brown, Yiling Lu, Nagendra K. Prasad, Bing Zhang, Gordon B. Mills, Wendell G. Yarbrough, Vito Quaranta, Motoharu Seiki, Alissa M. Weaver*

*To whom correspondence should be addressed. E-mail: alissa.weaver{at}vanderbilt.edu

This PDF file includes:

• Fig. S1. LY294002 treatment of SCC25 cells decreases invadopodia activity.
• Fig. S2. Average focal adhesion area data for Figs. 4A, 5B, and 6C and fig. S5D.
• Fig. S3. Western blot analyses and full image series data for Fig. 5.
• Fig. S4. Knockdown of PKCα in breast cancer and melanoma cells has an opposite effect on invadopodia activity depending on the PI3K status.
• Fig. S5. Inhibition of PKCα in SCC61 cells increases invadopodia activity.
• Fig. S6. PKCα knockdown leads to divergent Matrigel invasion phenotypes, depending on PI3K mutant status.
• Fig. S7. Visualizations of PRKCA and PIK3R1/PI(3,4,5)P₃/PI(3,4)P₂ binding interactions with focal adhesion and invadopodia proteins.
• Captions for Movies S1 to S6
  

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). Molecular focal adhesion and invadopodia network lists.
• Table S2 (Microsoft Excel format). PI-protein interaction lists.
• Table S3 (Microsoft Excel format). Random walk analysis results.
• Table S4 (Microsoft Excel format). RPPA analyte median centered data, patient data, analyte scores.
• Movies S1 to S6 (.mp4 and .mov format). Dynamics of focal adhesions and invadopodia in SCC25 cells expressing the H1047R PIK3CA mutant in the absence or presence of siRNA targeting PKCα.

© 2012 American Association for the Advancement of Science