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Sci. Signal., 18 September 2012
[DOI: 10.1126/scisignal.2002255]

Supplementary Materials for:

Charting the Landscape of Tandem BRCT Domain–Mediated Protein Interactions

Nicholas T. Woods, Rafael D. Mesquita, Michael Sweet, Marcelo A. Carvalho, Xueli Li, Yun Liu, Huey Nguyen, C. Eric Thomas, Edwin S. Iversen Jr., Sylvia Marsillac, Rachel Karchin, John Koomen, Alvaro N. A. Monteiro*

*To whom correspondence should be addressed. E-mail: alvaro.monteiro{at}moffitt.org

This PDF file includes:

  • Fig. S1. Literature curation PIN of BRCT-containing proteins.
  • Fig. S2. GO enrichment for the LIT Network.
  • Fig. S3. Enlarged and annotated 7X BRCT LIT subnetwork.
  • Fig. S4. GO enrichment for 7X BRCT LIT subnetwork.
  • Fig. S5. Y2H controls.
  • Fig. S6. Enlarged and annotated Y2H network.
  • Fig. S7. Mammalian-based validation of Y2H interactions.
  • Fig. S8. GO enrichment for the Y2H network.
  • Fig. S9. The effect of irradiation on the localization of tBRCT domain–containing proteins and protein-protein interactions.
  • Fig. S10. Enlarged and annotated TAP-MS network.
  • Fig. S11. GO enrichment for the TAP-MS network.
  • Fig. S12. Validation of interactions by reverse tagging.
  • Fig. S13. GO enrichment for the Merged Network.
  • Fig. S14. GO enrichment for the Merged Network and PIN clusters.
  • Fig. S15. COMMD1 knockdown enhances the apoptotic response of breast cancer cells treated with DNA-damaging agents.
  • Fig. S16. Comparison of the experimental designs and the top 10 enriched domains in data sets of this study and two others.
  • Fig. S17. The tBRCT disease network.
  • Fig. S18. Mutational analysis of tBRCT data sets using the COSMIC database.
  • Legends for Tables S1 to S12

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 25.3 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Human BRCT domains.
  • Table S2 (Microsoft Excel format). Literature-curated interactions with BRCT domain–containing proteins.
  • Table S3 (Microsoft Excel format). GO enrichment for the LIT network.
  • Table S4 (Microsoft Excel format). tBRCT-mediated interactions.
  • Table S5 (Microsoft Excel format). GO enrichment for the 7X BRCT LIT subnetwork.
  • Table S6 (Microsoft Excel format). Mammalian-based validation of interactions detected by Y2H assay and sensitivity of the interactions to phosphatase treatment.
  • Table S7 (Microsoft Excel format). GO enrichment for the Y2H network.
  • Table S8 (Microsoft Excel format). TAP-MS number of assigned spectra.
  • Table S9 (Microsoft Excel format). GO enrichment for the TAP-MS network.
  • Table S10 (Microsoft Excel format). GO enrichment for the Merged Network.
  • Table S11 (Microsoft Excel format). GO enrichments from TAP, Y2H, and LIT interaction data sets compared to the BRCT bait alone.
  • Table S12 (Microsoft Excel format). Genes from the Merged Network associated with enriched GO terms for Biological Process.

[Download Tables S1 to S12]

Technical Details

Format: Microsoft Excel

Size: 1.46 MB


Citation: N. T. Woods, R. D. Mesquita, M. Sweet, M. A. Carvalho, X. Li, Y. Liu, H. Nguyen, C. E. Thomas, E. S. Iversen Jr., S. Marsillac, R. Karchin, J. Koomen, A. N. A. Monteiro, Charting the Landscape of Tandem BRCT Domain–Mediated Protein Interactions. Sci. Signal. 5, rs6 (2012).

© 2012 American Association for the Advancement of Science


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