Phosphorylation of Cytohesin-1 by Fyn Is Required for Initiation of Myelination and the Extent of Myelination During Development

Junji Yamauchi,* Yuki Miyamoto, Tomohiro Torii, Shou Takashima, Kazumi Kondo, Katsumasa Kawahara, Noriko Nemoto, Jonah R. Chan, Gozoh Tsujimoto, Akito Tanoue

*To whom correspondence should be addressed. E-mail: jyamauchi{at}nch.go.jp

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• Fig. S1. Analysis of cytohesin-1 localization in cocultures and sciatic nerves.
• Fig. S2. Confirmation of the specificity of an affinity-purified antibody for cytohesin-1.
• Fig. S3. Effects of neuronal CM on the expression of cytohesin-family GEFs.
• Fig. S4. Specific precipitation of a constitutively active cytohesin-1 with guanine nucleotide–free Arf6.
• Fig. S5. Estimation of a purified cytohesin-1 protein.
• Fig. S6. Identification of the site in cytohesin-1 that is phosphorylated by Fyn.
• Fig. S7. Binding of cytohesin-1 to the SH1 domain of Fyn.
• Fig. S8. Confirmation of the specificity of an affinity-purified antibody against (pY³⁸²)cytohesin-1.
• Fig. S9. Comparison of the amino acid sequences of cytohesin-family GEFs.
• Fig. S10. Estimation of purified Y382F mutant and Arf6 proteins and the guanine nucleotide–releasing assay.
• Fig. S11. Generation of cytohesin-1^–/– mice.
• Fig. S12. Analysis of the abundances of Oct6 and Krox20 in sciatic nerves from cytohesin-1^–/– mice.
• Fig. S13. Analysis of cell numbers in sciatic nerves from cytohesin-1^–/– mice.
• Fig. S14. EM analysis of sciatic nerves from cytohesin-1^–/– mice.
• Fig. S15. Generation of transgenic mice (TG#1 and TG#5) of the Y382F mutant.
• Fig. S16. Analysis of the abundances of Oct6 and Krox20 in transgenic mice.
• Fig. S17. Analysis of cell numbers in sciatic nerves from transgenic mice.
• Fig. S18. EM analysis of sciatic nerves from transgenic mice.

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© 2012 American Association for the Advancement of Science