An Interaction Between BZR1 and DELLAs Mediates Direct Signaling Crosstalk Between Brassinosteroids and Gibberellins in Arabidopsis

Qian-Feng Li, Chunming Wang, Lei Jiang, Shuo Li, Samuel S. M. Sun,* Jun-Xian He*

*To whom correspondence should be addressed. E-mail: jxhe{at}cuhk.edu.hk (J.-X.H.); ssun{at}cuhk.edu.hk (S.S.M.S.)

This PDF file includes:

• Fig. S1. BES1 interacts with RGA in a yeast two-hybrid assay.
• Fig. S2. The LHR1 domain of RGA and the BIN2 phosphorylation domain of BZR1 mediate the interaction of RGA and BZR1.
• Fig. S3. BiFC analysis reveals a BES1 and RGA interaction in planta.
• Fig. S4. The gai mutant (with the GAIΔ17 mutation) partially suppresses root elongation of bzr1-1D.
• Fig. S5. RGA and GAI overexpression fails to alter the sensitivity of bzr1-1D to BRZ.
• Fig. S6. The gai mutation enhances the dwarf phenotypes of bri1-5 in a root elongation assay.
• Fig. S7. The RGA and GAI loss-of-function double mutant (rga-24 gai-t6) has increased sensitivity to BL but reduced sensitivity to BRZ.
• Fig. S8. The double mutant rga-24 gai-t6 has reduced sensitivity to BRZ in a hypocotyl elongation assay.
• Fig. S9. bzr1-1D reduces and bri1-5 enhances sensitivity to the GA inhibitor PAC.
• Fig. S10. bzr1-1D reduces and bri1-5 enhances sensitivity to PAC in a hypocotyl elongation assay.
• Fig. S11. DELLAs and BZR1 and BES1 have limited effects on each other's gene expression.
• Fig. S12. Quantitative analysis of the effect of GA or inhibition of GA biosynthesis or of BIN2 or PP2A activity on BZR1 abundance and hosphorylation status.
• Fig. S13. DELLAs inhibit growth of BZR1-overexpressing plants.
• Fig. S14. RGA and BIN2 do not interact.
• Fig. S15. RGA does not compete with PP2A for phosphorylated BZR1.
• Fig. S16. Inhibition of GA signaling stimulates the expression of PP2AB'α and PP2AB'β.
• Fig. S17. GA signaling inhibits SCL3 expression.
• Fig. S18. Some BZR1 target genes and RGAΔ17 responsive genes are common.
• Table S1. Primers used for plasmid construction in yeast two-hybrid assays.
• Table S2. Primers used for RT-PCR and qRT-PCR analyses.
• Table S3. Primers used for plasmid construction in other experiments.

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© 2012 American Association for the Advancement of Science