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Sci. Signal., 16 October 2012
[DOI: 10.1126/scisignal.2003086]

Supplementary Materials for:

TWEAK and cIAP1 Regulate Myoblast Fusion Through the Noncanonical NF-κB Signaling Pathway

Emeka K. Enwere, Janelle Holbrook, Rim Lejmi-Mrad, Jennifer Vineham, Kristen Timusk, Baktharaman Sivaraj, Methvin Isaac, David Uehling, Rima Al-awar, Eric LaCasse, Robert G. Korneluk*

*To whom correspondence should be addressed. E-mail: bob{at}

This PDF file includes:

  • Synthesis, structure, and characterization of the SMC OICR720.
  • Fig. S1. SMC triggers the loss of cIAP1 from primary myoblasts.
  • Fig. S2. The abundances of mitochondrial markers are not affected by loss of cIAP1.
  • Fig. S3. Loss of cIAP1 inhibits myoblast fusion in C2C12 cells.
  • Fig. S4. Loss of cIAP1 leads to activation of NF-κB pathways.
  • Fig. S5. TWEAK activates noncanonical NF-κB signaling and myoblast fusion.
  • Fig. S6. TWEAK activates canonical NF-κB signaling only at high concentrations.
  • Fig. S7. NEMO is not required for TWEAK-enhanced myoblast fusion.
  • Fig. S8. MEK1/2 inhibitors attenuate basal and TWEAK-enhanced myoblast fusion.
  • Table S1. Sequences of siRNA duplexes.
  • Movie S1 legend

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Format: Adobe Acrobat PDF

Size: 2.73 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Time-lapse imaging of myoblast fusion.

Technical Details


Size: 10.8 MB

Citation: E. K. Enwere, J. Holbrook, R. Lejmi-Mrad, J. Vineham, K. Timusk, B. Sivaraj, M. Isaac, D. Uehling, R. Al-awar, E. LaCasse, R. G. Korneluk, TWEAK and cIAP1 Regulate Myoblast Fusion Through the Noncanonical NF-κB Signaling Pathway. Sci. Signal. 5, ra75 (2012).

© 2012 American Association for the Advancement of Science

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