The Temporal Pattern of Stimulation Determines the Extent and Duration of MAPK Activation in a Caenorhabditis elegans Sensory Neuron

Taichiro Tomida, Shigekazu Oda, Mutsuhiro Takekawa, Yuichi Iino, Haruo Saito*

*To whom correspondence should be addressed. E-mail: h-saito{at}ims.u-tokyo.ac.jp

This PDF file includes:

• Fig. S1. ERKy, a specific probe for the ERK-family MAPK activity.
• Fig. S2. Phosphorylation of ERKy by the ERK-family MAPKs.
• Fig. S3. Defective chemotaxis to NaCl in mpk-1(n2521) mutant.
• Fig. S4. The microfluidic chamber.
• Fig. S5. Time course of the decay of ERKy FRET signal.
• Fig. S6. Detection of MAPK activation in ASER by immunostaining.
• Fig. S7. Time courses of MPK-1 activity in individual animals.
• Fig. S8. Requirement of the TAX-4 cation channel for Ca²⁺ response and MPK-1 activation.
• Fig. S9. Dampening of Ca²⁺ response in ASER by repetitive stimulation.

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© 2012 American Association for the Advancement of Science