Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 23 October 2012
[DOI: 10.1126/scisignal.2003199]

Supplementary Materials for:

Akt Phosphorylates the Transcriptional Repressor Bmi1 to Block Its Effects on the Tumor-Suppressing Ink4a-Arf Locus

Yan Liu,* Fan Liu, Hao Yu, Xinyang Zhao, Goro Sashida, Anthony Deblasio, Michael Harr, Qing-Bai She, Zhenbang Chen, Hui-Kuan Lin, Silvana Di Giandomenico, Shannon E. Elf, Youyang Yang, Yasuhiko Miyata, Gang Huang, Silvia Menendez, Ingo K. Mellinghoff, Neal Rosen, Pier Paolo Pandolfi, Cyrus V. Hedvat, Stephen D. Nimer*

*To whom correspondence should be addressed. E-mail: liu219{at}iupui.edu (Y.L.); snimer{at}med.miami.edu (S.D.N.)

This PDF file includes:

  • Fig. S1. Akt phosphorylates Bmi1 in cells.
  • Fig. S2. Knockdown of Bmi1 in wild-type MEFs with RNA interference.
  • Fig. S3. The Bmi1-S316A mutant enhances tumor formation in nude mice.
  • Fig. S4. Phosphorylated Bmi1 is detectable in human diffuse large B cell lymphoma samples.
  • Fig. S5. The PI3K-Akt signaling pathway does not affect PRC1 complex formation.
  • Fig. S6. Subcellular localization of Bmi1 in U2OS cells after serum or IGF exposure.
  • Fig. S7. ChIP assays of the Ink4a-Arf locus in MEFs.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 637 KB


Citation: Y. Liu, F. Liu, H. Yu, X. Zhao, G. Sashida, A. Deblasio, M. Harr, Q.-B. She, Z. Chen, H.-K. Lin, S. Di Giandomenico, S. E. Elf, Y. Yang, Y. Miyata, G. Huang, S. Menendez, I. K. Mellinghoff, N. Rosen, P. P. Pandolfi, C. V. Hedvat, S. D. Nimer, Akt Phosphorylates the Transcriptional Repressor Bmi1 to Block Its Effects on the Tumor-Suppressing Ink4a-Arf Locus. Sci. Signal. 5, ra77 (2012).

© 2012 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882