Mice Lacking the ITIM-Containing Receptor G6b-B Exhibit Macrothrombocytopenia and Aberrant Platelet Function

Alexandra Mazharian, Ying-Jie Wang, Jun Mori, Danai Bem, Brenda Finney, Silke Heising, Paul Gissen, James G. White, Michael C. Berndt, Elizabeth E. Gardiner, Bernhard Nieswandt, Michael R. Douglas, Robert D. Campbell, Steve P. Watson, Yotis A. Senis*

*To whom correspondence should be addressed. E-mail: y.senis{at}bham.ac.uk

This PDF file includes:

• Fig. S1. Comparison of human and mouse G6b-B.
• Fig. S2. Alignment of human and mouse G6b-B complementary DNA nucleotide sequences.
• Fig. S3. Biochemical comparison of mouse and human G6b-B.
• Fig. S4. G6b targeting strategy.
• Fig. S5. Analysis of G6b^–/– mice.
• Fig. S6. Analysis of G6b-B–deficient megakaryocytes.
• Fig. S7. Aberrant ultrastructure of G6b-B–deficient platelets.
• Fig. S8. Analysis of bleeding in G6b^–/– mice.
• Fig. S9. Surface glycoprotein abundance on G6b-B–deficient platelets.
• Fig. S10. Tyrosine phosphorylation in G6b-B–deficient platelets.
• Fig. S11. Responses of G6b-B–deficient platelets to collagen.
• Fig. S12. Reduced GPVI and GPIbα abundance in G6b^–/– bone marrow megakaryocytes.
• Fig. S13. Surface glycoprotein abundance on platelets from G6b/Gp6 DKO mice.
• Fig. S14. Antibody-induced depletion of CLEC-2 in wild-type mice.
• Fig. S15. Antibody-induced depletion of CLEC-2 in G6b/Gp6 DKO mice.
• Fig. S16. G6b-B/GPVI double-heterozygous mice have normal platelet counts and volumes.
• Fig. S17. Platelets from G6b-B/GPVI double-heterozygous mice respond better to collagen and CRP than do platelets from GPVI heterozygous mice.
• Table S1. Hematological analysis of G6b^–/– mice.
• Table S2. Hematological analysis of G6b/Gp6 DKO mice.

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© 2012 American Association for the Advancement of Science