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Sci. Signal., 6 November 2012
[DOI: 10.1126/scisignal.2003065]

Supplementary Materials for:

Proliferative and Antiapoptotic Signaling Stimulated by Nuclear-Localized PDK1 Results in Oncogenesis

Chintan K. Kikani, Erik V. Verona, Jiyoon Ryu, Yanying Shen, Qingqing Ye, Li Zheng, Ziliang Qian, Hiroshi Sakaue, Kyoko Nakamura, Jie Du, Qunsheng Ji, Wataru Ogawa, Lu-Zhe Sun, Lily Q. Dong, Feng Liu*

*To whom correspondence should be addressed. E-mail: liuf{at}

This PDF file includes:

  • Fig. S1. Characterization of cell lines expressing wild-type or nuclear-localized PDK1.
  • Fig. S2. MEFs with nuclear-localized PDK1 exhibit increased proliferation.
  • Fig. S3. Comparison of protein abundances and signaling in W-PDK1–/– cells.
  • Fig. S4. Nuclear localization of PDK1 increases resistance to apoptosis.
  • Fig. S5. Quantitative analysis of TNF-α–stimulated JNK1 activation and apoptosis in various PDK1-expressing cell lines.
  • Fig. S6. Suppression of the TNF-α–stimulated phosphorylation and nuclear translocation of the transcription factor c-Jun.
  • Fig. S7. Model depicting the regulation of the nuclear localization of PDK1 and its oncogenic function.
  • Table S1. Correlation of PDK1 subcellular localization with prostate cancer clinicopathological parameters.

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Citation: C. K. Kikani, E. V. Verona, J. Ryu, Y. Shen, Q. Ye, L. Zheng, Z. Qian, H. Sakaue, K. Nakamura, J. Du, Q. Ji, W. Ogawa, L.-Z. Sun, L. Q. Dong, F. Liu, Proliferative and Antiapoptotic Signaling Stimulated by Nuclear-Localized PDK1 Results in Oncogenesis. Sci. Signal. 5, ra80 (2012).

© 2012 American Association for the Advancement of Science

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