The Tetraspanin CD37 Orchestrates the α₄β₁ Integrin–Akt Signaling Axis and Supports Long-Lived Plasma Cell Survival

Annemiek B. van Spriel, Sandra de Keijzer, Alie van der Schaaf, Kate H. Gartlan, Mariam Sofi, Amanda Light, Peter C. Linssen, Jan B. Boezeman, Malou Zuidscherwoude, Inge Reinieren-Beeren, Alessandra Cambi, Fabienne Mackay, David M. Tarlinton, Carl G. Figdor,* Mark D. Wright

*To whom correspondence should be addressed. E-mail: c.figdor{at}ncmls.ru.nl

This PDF file includes:

• Methods
• Fig. S1. Production of IgG2b, IgG3, and IgM in Cd37^–/– mice.
• Fig. S2. Splenic B cell subsets in Cd37^–/– mice.
• Fig. S3. Decreased population of memory B cells in spleens from Cd37^–/– mice.
• Fig. S4. Normal proliferation of Cd37^–/– B cells.
• Fig. S5. Blocking the α₄ integrin reduces cell rolling and adhesion.
• Fig. S6. Cd37 expression in plasmablasts and plasma cells.
• Fig. S7. Impaired Akt phosphorylation in IgG1-secreting Cd37^–/– cells as detected by flow cytometry.
• Fig. S8. Average area of α₄β₁ integrin fluorescence at the VCAM-1 contact site.
• Movies S1 and S2 legends
  

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Rolling and adhesion of wild-type B cells under flow.
• Movie S2 (.mp4 format). Rolling and adhesion of Cd37^–/– B cells under flow.

© 2012 American Association for the Advancement of Science