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Sci. Signal., 4 December 2012
[DOI: 10.1126/scisignal.2003485]

Supplementary Materials for:

RNF4-Dependent Hybrid SUMO-Ubiquitin Chains Are Signals for RAP80 and Thereby Mediate the Recruitment of BRCA1 to Sites of DNA Damage

Catherine M. Guzzo, Christopher E. Berndsen, Jianmei Zhu, Vibhor Gupta, Ajit Datta, Roger A. Greenberg, Cynthia Wolberger, Michael J. Matunis*

*To whom correspondence should be addressed. E-mail: mmatunis{at}jhsph.edu

This PDF file includes:

  • Fig. S1. Multiple components of the BRCA1-A complex bind SUMO.
  • Fig. S2. Quantitative analysis of in vitro binding of GST-tagged RAP80 proteins to SUMO and ubiquitin.
  • Fig. S3. Analysis of the abundance of wild-type, UIM, and SIM mutant GFP-RAP80 fusion proteins expressed in U2OS cells.
  • Fig. S4. RAP80 binds with high affinity to hybrid SUMO-diubiquitin chains.
  • Fig. S5. RNF4 is required for RAP80 and BRCA1 recruitment to DSBs.
  • Fig. S6. Transfected U2OS cells express equivalent amounts of wild-type RNF4-GFP and RING mutant RNF4-GFP.

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Technical Details

Format: Adobe Acrobat PDF

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Citation: C. M. Guzzo, C. E. Berndsen, J. Zhu, V. Gupta, A. Datta, R. A. Greenberg, C. Wolberger, M. J. Matunis, RNF4-Dependent Hybrid SUMO-Ubiquitin Chains Are Signals for RAP80 and Thereby Mediate the Recruitment of BRCA1 to Sites of DNA Damage. Sci. Signal. 5, ra88 (2012).

© 2012 American Association for the Advancement of Science


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