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Sci. Signal., 4 December 2012
[DOI: 10.1126/scisignal.2003264]

Supplementary Materials for:

G Protein–Coupled Receptor–Mediated Activation of p110β by Gβγ Is Required for Cellular Transformation and Invasiveness

Hashem A. Dbouk, Oscar Vadas, Aliaksei Shymanets, John E. Burke, Rachel S. Salamon, Bassem D. Khalil, Mathew O. Barrett, Gary L. Waldo, Chinmay Surve, Christine Hsueh, Olga Perisic, Christian Harteneck, Peter R. Shepherd, T. Kendall Harden, Alan V. Smrcka, Ronald Taussig, Anne R. Bresnick, Bernd Nürnberg, Roger L. Williams,* Jonathan M. Backer*

*To whom correspondence should be addressed. E-mail: rlw{at}mrc-lmb.cam.ac.uk (R.L.W.); jonathan.backer{at}einstein.yu.edu (J.M.B.)

This PDF file includes:

  • Fig. S1. Sequence alignment of p110α, p110β, and p110δ.
  • Fig. S2. Mapping of the Gβγ-binding region on p110β at the membrane with HDX-MS.
  • Fig. S3. Mapping of the Gβγ-binding region in p110β with HDX-MS.
  • Fig. S4. In vitro stimulation of PI3Kβ mutants by Gβγ.
  • Fig. S5. Activation of Akt in cells transfected with plasmids encoding PI3Kβ and Gβγ: inhibition by TGX221 and p110β-derived peptide.
  • Fig. S6. Pertussis toxin–sensitive effects of PI3Kβ.
  • Fig. S7. Peptides derived from p110β inhibit GPCR-mediated activation of p110β signaling in intact cells.
  • Fig. S8. Peptide inhibitors of Gβγ-mediated PI3Kβ activation are specific for p85-p110β.
  • Fig. S9. In vitro activity of heterodimers of p110β associated with p85 truncation constructs.
  • Table S1. Summary of all p110β peptides analyzed by HDX-MS for the p110β–p85α-icSH2 dimer (wild type) and for the p110β–Gβ-p85α-icSH2–Gγ-C68S (fusion) complexes.
  • Table S2. Summary of all p85 regulatory subunit peptides analyzed by HDX-MS for the p110β–p85α-icSH2 dimer (wild type) and for the p110β–Gβ-p85α-icSH2–Gγ-C68S (fusion) complexes.
  • Table S3. Summary of all peptide exchange data for the Gβ subunit for HDX-MS experiments with wild-type and fusion complexes.
  • Table S4. Summary of all peptide exchange data for the Gγ subunit for HDX-MS experiments with wild-type and fusion complexes.
  • Table S5. Summary of all p110β peptides analyzed by HDX-MS for PI3Kβ-pY with and without liposomes and Gβγ complexes.
  • Table S6. Summary of all p85 regulatory subunit peptides analyzed by HDX-MS for the PI3Kβ-pY with and without liposomes and Gβγ complexes.

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Citation: H. A. Dbouk, O. Vadas, A. Shymanets, J. E. Burke, R. S. Salamon, B. D. Khalil, M. O. Barrett, G. L. Waldo, C. Surve, C. Hsueh, O. Perisic, C. Harteneck, P. R. Shepherd, T. K. Harden, A. V. Smrcka, R. Taussig, A. R. Bresnick, B. Nürnberg, R. L. Williams, J. M. Backer, G Protein–Coupled Receptor–Mediated Activation of p110β by Gβγ Is Required for Cellular Transformation and Invasiveness. Sci. Signal. 5, ra89 (2012).

© 2012 American Association for the Advancement of Science


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