Mechanism of Stretch-Induced Activation of the Mechanotransducer Zyxin in Vascular Cells

Sahana Suresh Babu, Agnieszka Wojtowicz, Marc Freichel, Lutz Birnbaumer, Markus Hecker,* Marco Cattaruzza

*To whom correspondence should be addressed. E-mail: hecker{at}physiologie.uni-hd.de

This PDF file includes:

• Fig. S1. Stretch-induced, zyxin-dependent gene expression.
• Fig. S2. Technical controls and standards.
• Fig. S3. Expression of natriuretic peptides by human umbilical vein endothelial cells.
• Fig. S4. Representative immunofluorescence analyses of samples used for quantitative representations.
• Fig. S5. Western blotting analysis of stretch-induced zyxin translocation from focal adhesions (cytosolic fraction) to the nucleus (nuclear fraction) in cells after subcellular fractionation.
• Fig. S6. Expression of TRP channels in endothelial cells.
• Fig. S7. TRPC3 or TRPC6 mediates stretch-induced zyxin activation.
• Fig. S8. Characterization of the effect of TRPC3 inhibition on stretch-induced responses.
• Fig. S9. Phosphorylation of the stretch-sensitive residue Ser¹⁴² is necessary for ANP-induced nuclear translocation of zyxin.
• Fig. S10. Wall tension–induced zyxin signaling in lung epithelial and bladder smooth muscle cells.
• Table S1. Synthetic oligonucleotides used for cloning and for real-time RT-PCR analysis.

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© 2012 American Association for the Advancement of Science