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Sci. Signal., 15 January 2013
[DOI: 10.1126/scisignal.2003305]

Supplementary Materials for:

A Genome-Wide siRNA Screen Reveals Positive and Negative Regulators of the NOD2 and NF-κB Signaling Pathways

Neil Warner, Aaron Burberry, Luigi Franchi, Yun-Gi Kim, Christine McDonald, Maureen A. Sartor, Gabriel Núñez*

*To whom correspondence should be addressed. E-mail: bclx{at}umich.edu

This PDF file includes:

  • Fig. S1. Characterization of the NOD2 reporter cell line used in the genome-wide siRNA screen.
  • Fig. S2. Layout of the assay plate for the genome-wide siRNA screen.
  • Fig. S3. Data normalization.
  • Fig. S4. Data distribution.
  • Fig. S5. Screening assay reproducibility.
  • Fig. S6. Screening assay robustness.
  • Fig. S7. MDP-induced NF-κB luminescence weakly correlates with cell viability.
  • Fig. S8. Cell viability analysis helps reduce false positives.
  • Fig. S9. Mass spectra of WBP11 and FUS peptides isolated in a biochemical screen for NOD2-interacting proteins.
  • Fig. S10. NCF1 is a positive regulator of NF-κB signaling.
  • Fig. S11. ZDHHC2 is a general regulator of NF-κB signaling.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 3.51 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Primary screen results.
  • Table S2 (Microsoft Excel format). Published NOD2-interacting proteins.
  • Table S3 (Microsoft Excel format). ConceptGen enrichment of top 700 positive regulators.
  • Table S4 (Microsoft Excel format). Hits supported by microarray studies.
  • Table S5 (Microsoft Excel format). Hits supported by proteomic studies.
  • Table S6 (Microsoft Excel format). Hits supported in the literature.
  • Table S7 (Microsoft Excel format). References for Crohn's disease GWAS hits.
  • Table S8 (Microsoft Excel format). 313 secondary validation hits list—annotation.
  • Table S9 (Microsoft Excel format). 313 secondary validation results.

[Download Tables S1-S9]

Technical Details

Format: Zip Archive

Size: 4.68 MB

 


Citation: N. Warner, A. Burberry, L. Franchi, Y.-G. Kim, C. McDonald, M. A. Sartor, G. Núñez, A Genome-Wide siRNA Screen Reveals Positive and Negative Regulators of the NOD2 and NF-κB Signaling Pathways. Sci. Signal. 6, rs3 (2013).

© 2013 American Association for the Advancement of Science


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