T Cell Activation Results in Conformational Changes in the Src Family Kinase Lck to Induce Its Activation

Anja Stirnweiss, Roland Hartig, Steffi Gieseler, Jonathan A. Lindquist, Peter Reichardt, Lars Philipsen, Luca Simeoni, Mateusz Poltorak, Camilla Merten, Werner Zuschratter, Yury Prokazov, Wolfgang Paster, Hannes Stockinger, Thomas Harder, Matthias Gunzer, Burkhart Schraven*

*To whom correspondence should be addressed. E-mail: Burkhart.Schraven{at}med.ovgu.de

This PDF file includes:

• Fig. S1. Two versus three lifetimes are needed to describe the fluorescence decay of an ECFP-EYFP FRET pair.
• Fig. S2. Localization of the biosensor CLckY-1 at the plasma membrane.
• Fig. S3. Localization of the biosensor in transfected Lck-deficient JCam1.6 cells.
• Fig. S4. Conjugate formation between transfected JCam1.6 cells expressing CLckY- 1 and mock-treated Raji B cells as measured with the QA detector in the FLIM setup.
• Fig. S5. Lck does not coimmunoprecipitate with Csk.

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© 2013 American Association for the Advancement of Science