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Sci. Signal., 26 March 2013
[DOI: 10.1126/scisignal.2003483]

Supplementary Materials for:

PUMA and BIM Are Required for Oncogene Inactivation–Induced Apoptosis

Gregory R. Bean, Yogesh Tengarai Ganesan, Yiyu Dong, Shugaku Takeda, Han Liu, Po M. Chan, Yafen Huang, Lewis A. Chodosh, Gerard P. Zambetti, James J.-D. Hsieh, Emily H.-Y. Cheng*

*Corresponding author. E-mail: chenge1{at}mskcc.org

This PDF file includes:

  • Fig. S1. Immunoblot analysis of siRNA-mediated knockdown of BIM and PUMA.
  • Fig. S2. Immunoblot analysis of siRNA-mediated knockdown.
  • Fig. S3. Regulation of ERK and AKT phosphorylation by constitutively active AKT and MEK.
  • Fig. S4. Inhibition of lapatinib-mediated induction of PUMA mRNA by Myr-AKT.
  • Fig. S5. Inhibition of ERK and AKT phosphorylation by various kinase inhibitors.
  • Fig. S6. PUMA is required for GDC0941-induced apoptosis of BT474 cells.
  • Fig. S7. BIM mRNA is induced upon treatment with tyrosine kinase inhibitors.
  • Fig. S8. Knockdown of p53 fails to prevent lapatinib-mediated PUMA induction and apoptosis.
  • Fig. S9. BIM and PUMA are activated upon erlotinib-induced apoptosis of PC9 cells.
  • Fig. S10. Immunoblot analysis of HER2 in HER2/Neu-driven mammary tumors.
  • Fig. S11. Immunoblot analysis of BIM and PUMA in HER2/Neu-driven mammary tumors.
  • Fig. S12. Immunoblot analysis of EGFR in the tetracycline-inducible EGFRL858R transgenic mouse model.
  • Fig. S13. Immunoblot analysis of PUMA in EGFRL858R-driven mouse lung tumors.
  • Fig. S14. Immunoblot analysis of phosphorylation of AKT and S6K.
  • Fig. S15. Immunoblot analysis of siRNA-mediated knockdown of PUMA.

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Citation: G. R. Bean, Y. T. Ganesan, Y. Dong, S. Takeda, H. Liu, P. M. Chan, Y. Huang, L. A. Chodosh, G. P. Zambetti, J. J.-D. Hsieh, E. H.-Y. Cheng, PUMA and BIM Are Required for Oncogene Inactivation–Induced Apoptosis. Sci. Signal. 6, ra20 (2013).

© 2013 American Association for the Advancement of Science


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