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Sci. Signal., 19 November 2013
[DOI: 10.1126/scisignal.2004518]

Supplementary Materials for:

Cytokinins Secreted by Agrobacterium Promote Transformation by Repressing a Plant Myb Transcription Factor

Nagesh Sardesai, Lan-Ying Lee, Huabang Chen, HoChul Yi, Gayla R. Olbricht, Alexandra Stirnberg, Jacob Jeffries, Kia Xiong, R.W. Doerge, Stanton B. Gelvin*

*Corresponding author. E-mail: gelvin{at}purdue.edu

This PDF file includes:

  • Fig. S1. Expression of MTF1 influences plant susceptibility to Agrobacterium-mediated transformation.
  • Fig. S2. Silencing MTF1 by RNAi in roots of Arabidopsis ecotype Bl-1 increases transformation susceptibility.
  • Fig. S3. Decreased transient transformation of Col-0 roots inoculated with miaA mutant A. tumefaciens.
  • Fig. S4. Phytohormone pretreatment of Arabidopsis roots increases susceptibility to Agrobacterium-mediated transformation.
  • Fig. S5. MTF1 expression is negatively regulated by cytokinin treatment.
  • Fig. S6. Genes encoding cytokinin signaling pathway proteins are involved in Agrobacterium-mediated transformation.
  • Fig. S7. Manipulation of Arabidopsis genes that are regulated by MTF1 increases susceptibility to Agrobacterium-mediated transformation.
  • Table S1. Microarray data.
  • Table S2. Sequences of primers used.

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Citation: N. Sardesai, L.-Y. Lee, H. Chen, H. Yi, G. R. Olbricht, A. Stirnberg, J. Jeffries, K. Xiong, R. W. Doerge, S. B. Gelvin, Cytokinins secreted by Agrobacterium promote transformation by repressing a plant Myb transcription factor. Sci. Signal. 6, ra100 (2013).

© 2013 American Association for the Advancement of Science


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