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Sci. Signal., 17 December 2013
[DOI: 10.1126/scisignal.2004560]

Supplementary Materials for:

Integration of Protein Abundance and Structure Data Reveals Competition in the ErbB Signaling Network

Christina Kiel,* Erik Verschueren, Jae-Seong Yang, Luis Serrano

*Corresponding author. E-mail: christina.kiel{at}crg.eu

This PDF file includes:

  • Fig. S1. Overlap of PPIs from different data sets.
  • Fig. S2. Domains mediating PPIs in the ErbB network and the Ras XOR motif.
  • Fig. S3. Representation of PPIs with single and multi-interface XOR hub proteins.
  • Fig. S4. Modeling of variations in protein abundance at XOR motifs.
  • Fig. S5. Tissue-specific mRNA expression of proteins in the Ras XOR motif.
  • Fig. S6. Abundance of RIN1, CRAF, and downstream targets in MCF-7 and HEK293 cells overexpressing RIN1 or RIN1-CAAX.
  • Fig. S7. Phosphorylation of CRAF, MEK, and ERK in MCF-7 and HEK293 cells.
  • Fig. S8. Recombinant proteins used for quantitative Western blot.
  • Fig. S9. Analysis of protein abundance in MCF-7 and HEK293 cells by Western blot.
  • Fig. S10. Analysis of quantitative Western blotting technique.
  • Table S4. Protein abundances in MCF-7 and HEK293 cells determined using quantitative Western blot.
  • Table S5. Reactions and rate constants used to model the RIN1-Ras-CRAF XOR motif.
  • Table S6. Initial protein abundances used to model the RIN1-Ras-CRAF XOR motif.
  • Table S7. List of tissue categories from BioGPS database.

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Technical Details

Format: Adobe Acrobat PDF

Size: 4.49 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Annotation of the initial ErbB network with PPI and GO terms.
  • Table S2 (Microsoft Excel format). Annotation of the ErbB network with binding affinities, structural information, domain predictions, and phosphorylation sites.
  • Table S3 (Microsoft Excel format). Analysis of competition at XOR nodes using Monte Carlo simulations.
  • Data file S1. ErbB network (Cytoscape file).
  • Data file S2. High-resolution ErbB network (PDF).
  • Data file S3. Computational model of the Ras XOR motif (XML file).
  • Data file S4. Information for MEK2 antibody (PDF).

[Download Tables S1 to S3 and Data Files S1 to S4]

 


Citation: C. Kiel, E. Verschueren, J.-S. Yang, L. Serrano, Integration of Protein Abundance and Structure Data Reveals Competition in the ErbB Signaling Network. Sci. Signal. 6, ra109 (2013).

© 2013 American Association for the Advancement of Science


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