Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 14 January 2014
[DOI: 10.1126/scisignal.2004331]

Supplementary Materials for:

Antipsychotics Activate mTORC1-Dependent Translation to Enhance Neuronal Morphological Complexity

Heather Bowling, Guoan Zhang, Aditi Bhattacharya, Luis M. Pérez-Cuesta, Katrin Deinhardt, Charles A. Hoeffer, Thomas A. Neubert, Wen-biao Gan, Eric Klann,* Moses V. Chao*

*Corresponding author. E-mail: eklann{at}cns.nyu.edu (E.K.); moses.chao{at}med.nyu.edu (M.V.C.)

This PDF file includes:

  • Fig. S1. Characterization of haloperidol-mediated pAkt response.
  • Fig. S2. Puromycin dosing and control experiments for genetic manipulations.
  • Fig. S3. Proteomic analysis of measured proteins.
  • Fig. S4. Haloperidol-induced proteins that react with a MAP2 antibody in mouse striatal neurons or rat striatal neurons in vitro.
  • Fig. S5. DARPP32 can label spines in DIV14 cultured striatal neurons.
  • Legends for tables S1 to S4
  • Table S5. Statistical significance for three biological replicates for selected proteins of interest.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.5 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Complete results of the 5-hour proteomic screen.
  • Table S2 (Microsoft Excel format). Complete results of the 48-hour proteomic screens.
  • Table S3 (Microsoft Excel format). Haloperidol-induced increases in proteins linked to mTORC1 signaling.
  • Table S4 (Microsoft Excel format). Candidate proteins from cytoskeleton, translation, mRNA, membrane, and release protein groups.

[Download Tables S1 to S4]

 


Citation: H. Bowling, G. Zhang, A. Bhattacharya, L. M. Pérez-Cuesta, K. Deinhardt, C. A. Hoeffer, T. A. Neubert, W.-b. Gan, E. Klann, M. V. Chao, Antipsychotics Activate mTORC1-Dependent Translation to Enhance Neuronal Morphological Complexity. Sci. Signal. 7, ra4 (2014).

© 2014 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882