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Sci. Signal., 14 January 2014
[DOI: 10.1126/scisignal.2004331]

Supplementary Materials for:

Antipsychotics Activate mTORC1-Dependent Translation to Enhance Neuronal Morphological Complexity

Heather Bowling, Guoan Zhang, Aditi Bhattacharya, Luis M. Pérez-Cuesta, Katrin Deinhardt, Charles A. Hoeffer, Thomas A. Neubert, Wen-biao Gan, Eric Klann,* Moses V. Chao*

*Corresponding author. E-mail: eklann{at} (E.K.); moses.chao{at} (M.V.C.)

This PDF file includes:

  • Fig. S1. Characterization of haloperidol-mediated pAkt response.
  • Fig. S2. Puromycin dosing and control experiments for genetic manipulations.
  • Fig. S3. Proteomic analysis of measured proteins.
  • Fig. S4. Haloperidol-induced proteins that react with a MAP2 antibody in mouse striatal neurons or rat striatal neurons in vitro.
  • Fig. S5. DARPP32 can label spines in DIV14 cultured striatal neurons.
  • Legends for tables S1 to S4
  • Table S5. Statistical significance for three biological replicates for selected proteins of interest.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.5 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Complete results of the 5-hour proteomic screen.
  • Table S2 (Microsoft Excel format). Complete results of the 48-hour proteomic screens.
  • Table S3 (Microsoft Excel format). Haloperidol-induced increases in proteins linked to mTORC1 signaling.
  • Table S4 (Microsoft Excel format). Candidate proteins from cytoskeleton, translation, mRNA, membrane, and release protein groups.

[Download Tables S1 to S4]


Citation: H. Bowling, G. Zhang, A. Bhattacharya, L. M. Pérez-Cuesta, K. Deinhardt, C. A. Hoeffer, T. A. Neubert, W.-b. Gan, E. Klann, M. V. Chao, Antipsychotics Activate mTORC1-Dependent Translation to Enhance Neuronal Morphological Complexity. Sci. Signal. 7, ra4 (2014).

© 2014 American Association for the Advancement of Science

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