Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 21 January 2014
[DOI: 10.1126/scisignal.2004822]

Supplementary Materials for:

Roquin-2 Promotes Ubiquitin-Mediated Degradation of ASK1 to Regulate Stress Responses

Takeshi Maruyama, Toshihiro Araki, Yosuke Kawarazaki, Isao Naguro, Susanne Heynen, Pedro Aza-Blanc, Ze'ev Ronai, Atsushi Matsuzawa, Hidenori Ichijo*

*Corresponding author. E-mail: ichijo{at}mol.f.u-tokyo.ac.jp

This PDF file includes:

  • Text
    Fig. S1. Experimental procedures for the detection of ASK1 degradation.
  • Fig. S2. Imaging-based system for the detection of ASK1 proteasomal degradation.
  • Fig. S3. Validation of candidate E3 ligases targeting ASK1 with independent siRNAs.
  • Fig. S4. Validation of candidate E3 ligases targeting ASK1 by Western blot.
  • Fig. S5. Characterization of candidate E3 ligases targeting ASK1.
  • Fig. S6. Proteasome-dependent ASK1 degradation.
  • Fig. S7. Ubiquitination of the C terminus of ASK1 by Roquin-2.
  • Fig. S8. Evaluation of involvement of related signaling molecules in pathogen resistance.
  • References (2729)

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 4.71 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1. Primary screen results and materials. (Excel file)
  • Table S2. Secondary screen results and materials. (Excel file)

[Download Tables S1 and S2]


Citation: T. Maruyama, T. Araki, Y. Kawarazaki, I. Naguro, S. Heynen, P. Aza-Blanc, Z. Ronai, A. Matsuzawa, H. Ichijo, Roquin-2 Promotes Ubiquitin-Mediated Degradation of ASK1 to Regulate Stress Responses. Sci. Signal. 7, ra8 (2014).

© 2014 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882