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Sci. Signal., 28 January 2014
[DOI: 10.1126/scisignal.2004497]

Supplementary Materials for:

STAT3 Induction of miR-146b Forms a Feedback Loop to Inhibit the NF-κB to IL-6 Signaling Axis and STAT3-Driven Cancer Phenotypes

Michael Xiang, Nicolai J. Birkbak, Vida Vafaizadeh, Sarah R. Walker, Jennifer E. Yeh, Suhu Liu, Yasmin Kroll, Mark Boldin, Konstantin Taganov, Bernd Groner, Andrea L. Richardson, David A. Frank*

*Corresponding author. E-mail: david_frank{at}dfci.harvard.edu

This PDF file includes:

  • Fig. S1. Doxycycline-inducible cell systems for miRNA screening and analyses of miR-146b regulation.
  • Fig. S2. STAT target gene expression across mouse mammary developmental stages.
  • Fig. S3. Western blotting of cells after cytokine stimulation.
  • Fig. S4. Characterization of miR-146b promoter methylation.
  • Fig. S5. Effects of miR-146b mimic and IL-6–neutralizing antibody on STAT3 phosphorylation and gene expression.
  • Fig. S6. Effect of miR-146b mimic and JAK inhibitor in SUM-159 cells.
  • Fig. S7. Analysis of triple-negative tumors versus other molecular subtypes in TCGA breast cancer cohort.
  • Table S1. Results of miRNA screen.
  • Table S2. Primers for qRT-PCR.

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Citation: M. Xiang, N. J. Birkbak, V. Vafaizadeh, S. R. Walker, J. E. Yeh, S. Liu, Y. Kroll, M. Boldin, K. Taganov, B. Groner, A. L. Richardson, D. A. Frank, STAT3 Induction of miR-146b Forms a Feedback Loop to Inhibit the NF-κB to IL-6 Signaling Axis and STAT3-Driven Cancer Phenotypes. Sci. Signal. 7, ra11 (2014).

© 2014 American Association for the Advancement of Science


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