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Sci. Signal., 11 February 2014
[DOI: 10.1126/scisignal.2004751]

Supplementary Materials for:

β1 Integrin Inhibition Elicits a Prometastatic Switch Through the TGFβ–miR-200–ZEB Network in E-Cadherin–Positive Triple-Negative Breast Cancer

Hoa H. Truong, Jiangling Xiong, Veerander P. S. Ghotra, Ella Nirmala, Lizette Haazen, Sylvia E. Le Dévédec, Hayri E. Balcioğlu, Shuning He, B. Ewa Snaar-Jagalska, Erno Vreugdenhil, John H. N. Meerman, Bob van de Water, Erik H. J. Danen*

*Corresponding author. E-mail: e.danen{at}lacdr.leidenuniv.nl

This PDF file includes:

  • Fig. S1. Quantification of single cell migration in 3D collagen gels.
  • Fig. S2. Pathways affected by β1 integrin knockdown from microarray data.
  • Fig. S3. Restored cohesion in 4T1-shβ1 cells after miR-200 expression.
  • Legend for table S1
  • Table S2. shRNA sequences.
  • Table S3. qPCR primer sequences.
  • Legends for movies S1 and S2

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.68 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Genes affected by β1 integrin knockdown from microarray data.
  • Movie S1 (.mp4 format). 4T1 spheroid collectively invading into 3D collagen matrix.
  • Movie S2 (.mp4 format). 4T1-shβ1 spheroid with individual cells migrating in 3D collagen matrix.

[Download Table S1]

[Download Movies S1 and S2]


Citation: H. H. Truong, J. Xiong, V. P. S. Ghotra, E. Nirmala, L. Haazen, S. E. Le Dévédec, H. E. Balcioğlu, S. He, B. E. Snaar-Jagalska, E. Vreugdenhil, J. H. N. Meerman, B. van de Water, E. H. J. Danen, β1 Integrin Inhibition Elicits a Prometastatic Switch Through the TGFβ–miR-200–ZEB Network in E-Cadherin–Positive Triple-Negative Breast Cancer. Sci. Signal. 7, ra15 (2014).

© 2014 American Association for the Advancement of Science


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