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Sci. Signal., 18 February 2014
[DOI: 10.1126/scisignal.2004785]

Supplementary Materials for:

The Adaptor Protein p66Shc Inhibits mTOR-Dependent Anabolic Metabolism

Mohamed A. Soliman,* Anas M. Abdel Rahman, Dudley A. Lamming, Kivanç Birsoy, Judy Pawling, Maria E. Frigolet, Huogen Lu, I. George Fantus, Adrian Pasculescu, Yong Zheng, David M. Sabatini, James W. Dennis,* Tony Pawson

*Corresponding author. E-mail: ma.soliman{at}utoronto.ca (M.A.S.); dennis{at}lunenfeld.ca (J.W.D.)

This PDF file includes:

  • Fig. S1. A volcano plot for the metabolomic analysis of HeLa cells stably expressing shRNA targeting GFP or p66Shc.
  • Fig. S2. Unsupervised principal components analysis for targeted metabolomic analysis of p66Shc KO and p66+ MEFs.
  • Fig. S3. The abundance of GLUT1 in p66Shc-competent and p66Shc-deficient HeLa cells and MEFs is similar.
  • Fig. S4. p66Shc inhibits de novo synthesis of nonessential amino acids.
  • Fig. S5. Effect of amino acid deprivation on serum-mediated activation of mTORC1 in p66Shc-deficient cells.
  • Fig. S6. Effect of p66Shc expression on cell size.
  • Fig. S7. Effect of Akt inhibition on the amounts of glycolytic metabolites in p66Shc-competent and p66Shc-deficient MEFs.
  • Legend for table S1
  • Table S2. LC-MS/MS transitions for [1,2-13C2]glucose intermediates.
  • Table S3. List of genes showing differential regulation upon p66Shc expression.
  • Table S4. List of identified p66Shc-interacting proteins.

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Technical Details

Format: Adobe Acrobat PDF

Size: 834 KB

Other Supplementary Material for this manuscript includes the following:

  • Table S1. LC-MS/MS transitions for the metabolites measured in this study (Excel file).

[Download Table S1]


Citation: M. A. Soliman, A. M. Abdel Rahman, D. A. Lamming, K. Birsoy, J. Pawling, M. E. Frigolet, H. Lu, I. G. Fantus, A. Pasculescu, Y. Zheng, D. M. Sabatini, J. W. Dennis, T. Pawson, The Adaptor Protein p66Shc Inhibits mTOR-Dependent Anabolic Metabolism. 7, ra17 (2014).

© 2014 American Association for the Advancement of Science


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