A movie showing differentation of cultured cerebellar granule neurons and static microscopy images showing ceramide-treated or ceramide and PACAP-treated cerebellar granule neurons in culture were provided by D. Vaudry.
Movie. Differentiation of cultured cerebellar granule neurons. Image acquisition was started 3 hours after plating the cells and one image was collected every 5 minutes over a period of 12 hours using a Leica DMIRBE equiped with a heating chamber. The images were compiled into a movie, which plays at 8938x normal speed.
Microscopy Images.Ceramide and PACAP-treated cerebellar granule neurons. The effect of C2-ceramide and PACAP on cultured cerebellar granule neurons is shown. After 24 hours in the presence of ceramide (20 mM), most granule neurons exhibit the typical shape of apoptotic cells with a shrinked body and retracted neurites (left). Cells treated with 20 mM c2-ceramide plus 100 nM PACAP appear to be more numerous and exhibit a dense neurite network of healthy bipolar granule neurons (right).
S. Leuillet, D. Vaudry, A. Falluel-Morel, H. Vaudry, B. J. Gonzalez, Granule cell survival pathway. Sci. STKE (Connections Maps). http://stke.sciencemag.org/cgi/cm/CMP_11486. [Specific Pathway]
Citation for the movie: S. Leuillet, D. Vaudry, A. Falluel-Morel, H. Vaudry, B. J. Gonzalez, Cultured cerebellar granule cells movie. Sci. STKE (Supplement to Connections Maps), http://stke.sciencemag.org/cgi/cm/CMP_11486/DC1.
The movie is in Microsoft AVI format and viewable using Windows Media Player, which can be downloaded from Windows Media site.
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