A movie showing neurite extension of pheochromocytoma PC12 cells after treatment with pituitary adenylate cyclase-activating polypeptide (PACAP) was provided by D. Vaudry.
Movie 1. Stimulation of neurite outgrowth in PC12 cells after treatment with PACAP. Before imaging, the cells were treated for 2 days with PACAP and the protein kinase C (PKC) stimulator phorbol myristate acetate (PMA). The cells were then washed in fresh medium to remove PACAP and PKC, and image acquisition was started 15 minutes after the last wash. One image was collected every 3.33 minutes over a period of 12 hours using a Leica DMIRBE equipped with a heating chamber and a 5% CO2 supply. The images were compiled into a movie, which plays at 5972x normal speed.
D. Vaudry, P. J. S. Stork, P. Lazarovici, L. E. Eiden, Differentiation pathway in PC12 cells. Sci. STKE (Connections Maps). http://stke.sciencemag.org/cgi/cm/CMP_8038. [Specific Pathway]
Citation for the movie: D. Vaudry, Stimulation of neurite outgrowth in PC12 cells after treatment with PACAP. Sci. STKE (Supplement to Connections Maps), http://stke.sciencemag.org/cgi/cm/CMP_8038/DC1.
The movie is in Microsoft AVI format and viewable using Windows Media Player, which can be downloaded from Windows Media site.