Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. STKE, 31 May 2005
Vol. 2005, Issue 286, p. re7
[DOI: 10.1126/stke.2862005re7]


The Many Faces of SAM

Feng Qiao and James U. Bowie*

University of California, Los Angeles–U.S. Department of Energy (UCLA-DOE) Institute of Genomics and Proteomics, Molecular Biology Institute, Department of Chemistry and Biochemistry, UCLA, CA 90095, USA.

Gloss: Cells need to make appropriate responses to complex signals. One way in which they accomplish this task is by constructing large complexes of macromolecules, bringing together various sensors and effectors that facilitate the integration of input and output. Proteins are often brought into these macromolecular complexes by small modules that have specialized binding properties. The process of evolution can move these modules to different proteins, thereby conferring the distinctive binding properties of the module to new proteins. One particularly versatile protein module is called a SAM domain. SAM domains can associate into long strings containing many SAM domains or into small clusters of only a few SAM domains; they can bind to many different proteins, and they can also bind to RNA. It seems likely that some SAM domains could also provide functions that we do not yet know about. Efforts to learn about these additional functions continue. Ultimately, we would like to know how to predict the function of any SAM domain.

*Corresponding author. Department of Chemistry and Biochemistry, Room 655, Boyer Hall, UCLA, 611 Charles E. Young Drive East, Los Angeles, CA 90095–1570, USA. E-mail: bowie{at}

Citation: F. Qiao, J. U. Bowie, The Many Faces of SAM. Sci. STKE 2005, re7 (2005).

A cascade of ER exit site assembly that is regulated by p125A and lipid signals.
D. Klinkenberg, K. R. Long, K. Shome, S. C. Watkins, and M. Aridor (2014)
J. Cell Sci. 127, 1765-1778
   Abstract »    Full Text »    PDF »
Phosphorylation of the Usher syndrome 1G protein SANS controls Magi2-mediated endocytosis.
K. Bauss, B. Knapp, P. Jores, R. Roepman, H. Kremer, E. v. Wijk, T. Marker, and U. Wolfrum (2014)
Hum. Mol. Genet.
   Abstract »    Full Text »    PDF »
The composition of EphB2 clusters determines the strength in the cellular repulsion response.
A. Schaupp, O. Sabet, I. Dudanova, M. Ponserre, P. Bastiaens, and R. Klein (2014)
J. Cell Biol. 204, 409-422
   Abstract »    Full Text »    PDF »
SLP-76 Sterile {alpha} Motif (SAM) and Individual H5 {alpha} Helix Mediate Oligomer Formation for Microclusters and T-cell Activation.
H. Liu, Y. R. Thaker, L. Stagg, H. Schneider, J. E. Ladbury, and C. E. Rudd (2013)
J. Biol. Chem. 288, 29539-29549
   Abstract »    Full Text »    PDF »
Mixed Lineage Kinase-c-Jun N-Terminal Kinase Axis: A Potential Therapeutic Target in Cancer.
A. Rana, B. Rana, R. Mishra, G. Sondarva, V. Rangasamy, S. Das, N. Viswakarma, and A. Kanthasamy (2013)
Genes & Cancer 4, 334-341
   Abstract »    Full Text »    PDF »
The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells.
E. Franzolin, G. Pontarin, C. Rampazzo, C. Miazzi, P. Ferraro, E. Palumbo, P. Reichard, and V. Bianchi (2013)
PNAS 110, 14272-14277
   Abstract »    Full Text »    PDF »
Mutation in PHC1 implicates chromatin remodeling in primary microcephaly pathogenesis.
S. Awad, M. S. Al-Dosari, N. Al-Yacoub, D. Colak, M. A. Salih, F. S. Alkuraya, and C. Poizat (2013)
Hum. Mol. Genet. 22, 2200-2213
   Abstract »    Full Text »    PDF »
The Relationship Between Long-Range Chromatin Occupancy and Polymerization of the Drosophila ETS Family Transcriptional Repressor Yan.
J. L. Webber, J. Zhang, L. Cote, P. Vivekanand, X. Ni, J. Zhou, N. Negre, R. W. Carthew, K. P. White, and I. Rebay (2013)
Genetics 193, 633-649
   Abstract »    Full Text »    PDF »
A CC-SAM, for Coiled Coil-Sterile {alpha} Motif, Domain Targets the Scaffold KSR-1 to Specific Sites in the Plasma Membrane.
D. Koveal, N. Schuh-Nuhfer, D. Ritt, R. Page, D. K. Morrison, and W. Peti (2012)
Science Signaling 5, ra94
   Abstract »    Full Text »    PDF »
The Growth-Suppressive Function of the Polycomb Group Protein Polyhomeotic Is Mediated by Polymerization of Its Sterile Alpha Motif (SAM) Domain.
A. K. Robinson, B. Z. Leal, L. V. Chadwell, R. Wang, U. Ilangovan, Y. Kaur, S. E. Junco, V. Schirf, P. A. Osmulski, M. Gaczynska, et al. (2012)
J. Biol. Chem. 287, 8702-8713
   Abstract »    Full Text »    PDF »
Structures of Usher Syndrome 1 Proteins and Their Complexes.
L. Pan and M. Zhang (2012)
Physiology 27, 25-42
   Abstract »    Full Text »    PDF »
Differential regulation of the activity of deleted in liver cancer 1 (DLC1) by tensins controls cell migration and transformation.
X. Cao, C. Voss, B. Zhao, T. Kaneko, and S. S.-C. Li (2012)
PNAS 109, 1455-1460
   Abstract »    Full Text »    PDF »
A Sterile {alpha}-Motif Domain in NafY Targets Apo-NifDK for Iron-Molybdenum Cofactor Delivery via a Tethered Domain.
J. A. Hernandez, A. H. Phillips, W. K. Erbil, D. Zhao, M. Demuez, C. Zeymer, J. G. Pelton, D. E. Wemmer, and L. M. Rubio (2011)
J. Biol. Chem. 286, 6321-6328
   Abstract »    Full Text »    PDF »
FliZ Regulates Expression of the Salmonella Pathogenicity Island 1 Invasion Locus by Controlling HilD Protein Activity in Salmonella enterica Serovar Typhimurium.
J. E. C. Chubiz, Y. A. Golubeva, D. Lin, L. D. Miller, and J. M. Slauch (2010)
J. Bacteriol. 192, 6261-6270
   Abstract »    Full Text »    PDF »
Comparative Analysis of Chromatin Binding by Sex Comb on Midleg (SCM) and Other Polycomb Group Repressors at a Drosophila Hox Gene.
L. Wang, N. Jahren, E. L. Miller, C. S. Ketel, D. R. Mallin, and J. A. Simon (2010)
Mol. Cell. Biol. 30, 2584-2593
   Abstract »    Full Text »    PDF »
The SAM Domains of Anks Family Proteins Are Critically Involved in Modulating the Degradation of EphA Receptors.
J. Kim, H. Lee, Y. Kim, S. Yoo, E. Park, and S. Park (2010)
Mol. Cell. Biol. 30, 1582-1592
   Abstract »    Full Text »    PDF »
Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain.
S. M. Di Pietro, D. Cascio, D. Feliciano, J. U. Bowie, and G. S. Payne (2010)
EMBO J. 29, 1033-1044
   Abstract »    Full Text »    PDF »
{beta}Pix Up-regulates Na+/H+ Exchanger 3 through a Shank2-mediated Protein-Protein Interaction.
J.-S. Lee, Y. M. Lee, J. Y. Kim, H. W. Park, S. Grinstein, J. Orlowski, E. Kim, K. H. Kim, and M. G. Lee (2010)
J. Biol. Chem. 285, 8104-8113
   Abstract »    Full Text »    PDF »
The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins.
J. Yan, L. Pan, X. Chen, L. Wu, and M. Zhang (2010)
PNAS 107, 4040-4045
   Abstract »    Full Text »    PDF »
Involvement of Vts1, a structure-specific RNA-binding protein, in Okazaki fragment processing in yeast.
C.-H. Lee, Y.-K. Shin, T. T. H. Phung, J. S. Bae, Y.-H. Kang, T. A. Nguyen, J.-H. Kim, D.-H. Kim, M.-J. Kang, S.-H. Bae, et al. (2010)
Nucleic Acids Res. 38, 1583-1595
   Abstract »    Full Text »    PDF »
Aicardi-Goutieres syndrome and related phenotypes: linking nucleic acid metabolism with autoimmunity.
Y. J. Crow and J. Rehwinkel (2009)
Hum. Mol. Genet. 18, R130-R136
   Abstract »    Full Text »    PDF »
The importance of Src homology 2 domain-containing leukocyte phosphoprotein of 76 kilodaltons sterile-{alpha} motif domain in thymic selection and T-cell activation.
S. Shen, J. Lau, M. Zhu, J. Zou, D. Fuller, Q.-j. Li, and W. Zhang (2009)
Blood 114, 74-84
   Abstract »    Full Text »    PDF »
MAPKAP kinase MK2 maintains self-renewal capacity of haematopoietic stem cells.
J. Schwermann, C. Rathinam, M. Schubert, S. Schumacher, F. Noyan, H. Koseki, A. Kotlyarov, C. Klein, and M. Gaestel (2009)
EMBO J. 28, 1392-1406
   Abstract »    Full Text »    PDF »
EphA1 interacts with integrin-linked kinase and regulates cell morphology and motility.
T. Yamazaki, J. Masuda, T. Omori, R. Usui, H. Akiyama, and Y. Maru (2009)
J. Cell Sci. 122, 243-255
   Abstract »    Full Text »    PDF »
Effects of Structure of Rho GTPase-activating Protein DLC-1 on Cell Morphology and Migration.
T. Y. Kim, K. D. Healy, C. J. Der, N. Sciaky, Y.-J. Bang, and R. L. Juliano (2008)
J. Biol. Chem. 283, 32762-32770
   Abstract »    Full Text »    PDF »
Downregulation of Vertebrate Tel (ETV6) and Drosophila Yan Is Facilitated by an Evolutionarily Conserved Mechanism of F-Box-Mediated Ubiquitination.
M. G. Roukens, M. Alloul-Ramdhani, S. Moghadasi, M. Op den Brouw, and D. A. Baker (2008)
Mol. Cell. Biol. 28, 4394-4406
   Abstract »    Full Text »    PDF »
Identification of a New Site of Sumoylation on Tel (ETV6) Uncovers a PIAS-Dependent Mode of Regulating Tel Function.
M. G. Roukens, M. Alloul-Ramdhani, A. C. O. Vertegaal, Z. Anvarian, C. I. A. Balog, A. M. Deelder, P. J. Hensbergen, and D. A. Baker (2008)
Mol. Cell. Biol. 28, 2342-2357
   Abstract »    Full Text »    PDF »
Composition of the Synaptic PSD-95 Complex.
A. Dosemeci, A. J. Makusky, E. Jankowska-Stephens, X. Yang, D. J. Slotta, and S. P. Markey (2007)
Mol. Cell. Proteomics 6, 1749-1760
   Abstract »    Full Text »    PDF »
Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli.
M. L. Galisteo, Y. Yang, J. Urena, and J. Schlessinger (2006)
PNAS 103, 9796-9801
   Abstract »    Full Text »    PDF »
A KSR/CNK complex mediated by HYP, a novel SAM domain-containing protein, regulates RAS-dependent RAF activation in Drosophila..
M. Douziech, M. Sahmi, G. Laberge, and M. Therrien (2006)
Genes & Dev. 20, 807-819
   Abstract »    Full Text »    PDF »
An Architectural Framework That May Lie at the Core of the Postsynaptic Density.
M. K. Baron, T. M. Boeckers, B. Vaida, S. Faham, M. Gingery, M. R. Sawaya, D. Salyer, E. D. Gundelfinger, and J. U. Bowie (2006)
Science 311, 531-535
   Abstract »    Full Text »    PDF »
Mae inhibits Pointed-P2 transcriptional activity by blocking its MAPK docking site.
F. Qiao, B. Harada, H. Song, J. Whitelegge, A. J. Courey, and J. U. Bowie (2006)
EMBO J. 25, 70-79
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882