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Sci. Signal., 9 September 2008
[DOI: 10.1126/scisignal.1161577]

Phosphoinositide 3-Kinase p110β Activity: Key Role in Metabolism and Mammary Gland Cancer but Not Development

Elisa Ciraolo1, Manuela Iezzi2, Romina Marone3, Stefano Marengo1, Claudia Curcio4, Carlotta Costa1, Ornella Azzolino1, Cristiano Gonella1, Cristina Rubinetto1, Haiyan Wu5, Walter Dastrù6, Erica L. Martin7, Lorenzo Silengo1, Fiorella Altruda1, Emilia Turco1, Letizia Lanzetti8, Piero Musiani2, Thomas Rückle9, Christian Rommel9, Jonathan M. Backer5, Guido Forni4, Matthias P. Wymann3, and Emilio Hirsch1*

1Department of Genetics, Biology and Biochemistry, Molecular Biotechnology Center, University of Torino, Via Nizza 52, 10126 Torino, Italy.

2Aging Research Centre, G. d’Annunzio University Foundation, 66013 Chieti, Italy.

3Department of Biomedicine, Institute of Biochemistry and Genetics, University of Basel, Mattenstrasse 28, CH-4058 Basel, Switzerland.

4Department of Clinical and Biological Sciences, Molecular Biotechnology Center, University of Torino, via Nizza 52, 10126 Torino, Italy.

5Department of Molecular Pharmacology, Albert Einstein College of Medicine, 10461 Bronx, NY, USA.

6Department of Chemistry IFM, Molecular Imaging Center, University of Torino, Via Nizza 52, 10126 Torino, Italy.

7Department of Anesthesia and Critical Care, University of Torino, Corso Dogliotti 14, 10126 Torino, Italy.

8Dipartimento di Scienze Oncologiche, Istituto per la Ricerca e la Cura del Cancro, University of Torino, Strada Provinciale 142, 10060 Candiolo, Torino, Italy.

9Merck Serono S.A., Geneva Research Center, CH-1202 Geneva, Switzerland.

This PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Description of the gene targeting strategy
  • Fig. S2. Expression analysis of the products of the PIK3CBK805R allele
  • Fig. S3. Expression of PIK3CBK805R/K805R does not affect abundance of free p85
  • Fig. S4. Pharmacological inhibition of p110β by TGX-155 does not impair proliferation of wild-type MEFs
  • Fig. S5. Transfection of PIK3CBK805R/K805R low MEFs with Rab5Q79L constitutively active mutant does not rescue defective endocytosis of ligandbound EGFR
  • Fig. S6. Analysis of Akt phosphorylation by EGF or PDGF
  • Fig. S7. Body size and protein expression in adult PIK3CBK805R/K805R mice
  • Fig. S8. Role of PI3Kβ downstream of the insulin receptor in human hepatocellular liver carcinoma cell line (HEPG2)
  • Fig. S9. Tumor multiplicity and growth in PIK3CBWT/WT/neuT and PIK3CBK805R/K805R/neuT mice
  • Fig. S10. Homogeneous epithelial characteristics in both mutant and wild-type tumor-derived mammary gland cancer cell lines
  • Supplementary References

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*To whom correspondence should be addressed. E-mail: emilio.hirsch{at}unito.it

Citation: E. Ciraolo, M. Iezzi, R. Marone, S. Marengo, C. Curcio, C. Costa, O. Azzolino, C. Gonella, C. Rubinetto, H. Wu, W. Dastrù, E. L. Martin, L. Silengo, F. Altruda, E. Turco, L. Lanzetti, P. Musiani, T. Rückle, C. Rommel, J. M. Backer, G. Forni, M. P. Wymann, E. Hirsch, Phosphoinositide 3-Kinase p110β Activity: Key Role in Metabolism and Mammary Gland Cancer but Not Development. Sci. Signal. 1, ra3 (2008).

© 2008 American Association for the Advancement of Science


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