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Sci. Signal., 14 October 2008
[DOI: 10.1126/scisignal.1162329]

Regulation of P2X2 Receptors by the Neuronal Calcium Sensor VILIP1

Severine Chaumont,1,2,3,4,5* Vincent Compan,3,4,5 Estelle Toulme,1,2 Esther Richler,1,2 Gary D. Housley,6 Francois Rassendren,3,4,5 Baljit S. Khakh1,2*†

1Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.

2Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.

3CNRS, UMR 5203, Institut de Génomique fonctionnelle, Montpellier, France.

4INSERM, U661, Montpellier, France.

5Université Montpellier, 1 and 2, 34094 Montpellier, France.

6Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, NSW 2052 Australia.

*These authors contributed equally to experiments in this work.

This PDF file includes:

  • Materials and Methods
  • Fig. S1. The constitutive interaction between P2X2 receptors and VILIP1 is calcium independent.
  • Fig. S2. Colocalization between P2X2 receptors and VILIP1.
  • Fig. S3. ATP concentration–response curve for HEK cells expressing P2X2 receptors and VILIP-YFP, and dialyzed with BAPTA.
  • Fig. S4. Responses evoked by repetitive ATP application.
  • Fig. S5. Effects of VILIP1 on P2X2 receptor function.
  • Fig. S6. FRET efficiency determination between P2X2-CFP receptors and VILIP1-YFP in the absence of calcium
  • Fig. S7. Calcium entry independently of P2X2 receptors does not lead to elevated FRET between P2X2-CFP and VILIP1-YFP.
  • Table S1. Proteomic analysis of proteins interacting with the carboxyl-terminal cytoplasmic region of the P2X2 receptor.
  • References

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Technical Details

Format: Adobe Acrobat PDF

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†To whom correspondence should be addressed. E-mail: bkhakh{at}mednet.ucla.edu

Citation: S. Chaumont, V. Compan, E. Toulme, E. Richler, G. D. Housley, F. Rassendren, B. S. Khakh, Regulation of P2X2 receptors by the neuronal calcium sensor VILIP1. Sci. Signal. 1, ra8 (2008).

© 2008 American Association for the Advancement of Science


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