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Sci. Signal., 28 October 2008
[DOI: 10.1126/scisignal.1159665]

Supplementary Materials for
Essential Role of DAP12 Signaling in Macrophage Programming into a Fusion-Competent State

Laura Helming,1* Elena Tomasello,2,3,4 Themis R. Kyriakides,5,6,7 Fernando O. Martinez,1 Toshiyuki Takai,8,9 Siamon Gordon,1† Eric Vivier2,3,4,10†‡

1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.

2Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, case 906, Campus de Luminy, 13288 Marseille, France.

3INSERM, U631, case 906, Campus de Luminy, 13288 Marseille, France.

4CNRS,UMR6102, case 906, Campus de Luminy, 13288 Marseille, France.

5Vascular Biology and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.

6Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, USA.

7Department of Biomedical Engineering, Yale University School of Medicine, New Haven, CT 06520, USA.

8Department of Experimental Immunology, Institute of Development, Aging and Cancer, Tohoku University, Seiryo 4-1, Aoba-ku, Sendai 980-8575, Japan.

9Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan.

10Assistance Publique-Hôpitaux de Marseille, Hôpital de la Conception, 13385 Marseille, France.

*Present address: Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstrasse 30, 81675 Munich, Germany.

This PDF file includes:

  • Table S1. Abundance of surface CD11b is normal in DAP12-KI macrophages.
  • Fig. S1. Macrophage distribution is normal in organs of DAP12-KI mice.
  • Fig. S2. Macrophage fusion and expression of fusion mediators are reduced in DAP12-KI BMM.
  • Fig. S3. Reduced expression of fusion mediators in DAP12-KI macrophages on different tissue culture surfaces.
  • Fig. S4. Pangenomic transcription analysis reveals global changes in DAP12-KI macrophages.

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†To whom correspondence should be addressed. E-mail: siamon.gordon{at}path.ox.ac.uk (S.G.) and vivier{at}ciml.univ-mrs.fr (E.V.)

‡Joint senior authors.

Citation: L. Helming, E. Tomasello, T. R. Kyriakides, F. O. Martinez, T. Takai, S. Gordon, E. Vivier, Essential Role of DAP12 Signaling in Macrophage Programming into a Fusion-Competent State. Sci. Signal. 1, ra11 (2008).

© 2008 American Association for the Advancement of Science


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