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Sci. Signal., 11 November 2008
[DOI: 10.1126/scisignal.2000037]

Supplementary Materials for
New Regulators of Wnt/β-Catenin Signaling Revealed by Integrative Molecular Screening

Michael B. Major,1* Brian S. Roberts,2* Jason D. Berndt,1 Shane Marine,3 Jamie Anastas,1 Namjin Chung,3 Marc Ferrer,3 XianHua Yi,4 Cristi L. Stoick-Cooper,1 Priska D. von Haller,5 Lorna Kategaya,1 Andy Chien,1 Stephane Angers,6 Michael MacCoss,4 Michele A. Cleary,2 William T. Arthur,2† Randall T. Moon1†

1Howard Hughes Medical Institute, Department of Pharmacology, and Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Box 357370, Seattle, WA 98195, USA.

2Rosetta Inpharmatics, LLC, Merck & Co Inc., 401 Terry Avenue N, Seattle, WA 98109, USA.

3Merck Research Labs, Department of Automated Biotechnology, North Wales, PA 19454, USA.

4Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195, USA.

5Proteomics Resource, University of Washington, Seattle, WA 98109, USA.

6Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada M5S 3M2.

Supplementary Materials (PDF File)

  • Fig. S1. Scalable vector graphic representation of the integration of siRNA screen hits on protein-protein interaction networks.
  • Fig. S2. AGGF1 is required both for the expression of LEF1 and AXIN2 and associates with the LEF1 and AXIN2 promoters.
  • Fig. S3. Recombinant human AGGF1 (rhAGGF1) does not regulate β-catenin-mediated transcription when added to the media of DLD1 cells.

Format: Adobe Acrobat PDF

Size: 5,508 KB

[Download PDF]

Supplementary Materials (Excel Files)

  • Table S1. Genome-wide primary siRNA screen data from DLD1 cells.
  • Table S2. Genome-wide secondary siRNA screen data from DLD1 cells.
  • Table S3. Pilot-scale secondary siRNA screen data from DLD1 cells.
  • Table S4. Genome-wide secondary siRNA screen data from SW480 cells.
  • Table S5. Pilot-scale secondary siRNA screen data from SW480 cells.
  • Table S6. Compiled siRNA screen hits that passed the secondary screen.
  • Table S7. Tertiary screen Fluidigm real-time PCR data from DLD1 cells.
  • Table S8. Tertiary screen cDNA microarray data from DLD1 cells.
  • Table S9. 38 hits that passed tertiary screen.
  • Table S10. Workbook containing filtered and compiled TAP-MS data.
  • Table S11. STRING-based protein-protein interaction data for core components of the Wnt pathway.
  • Table S12. Statistics on the specificity of the siRNA screen hits to the Wnt pathway and on the interconnectivity of proteins identified in the screen.
  • Table S13. STRING-based protein-protein interaction data for siRNA screen hits.
  • Table S14. Genes regulated by both β-catenin and AGGF1.
  • Table S15. AGGF1 and BAF57 expression in colon cancer.
  • Table S16. 112 genes identified by siRNA screens and proteomic interaction maps.
  • Table S17. Evolutionarily conserved modulators of Wnt/β-catenin signaling.
  • Table S18. Reagents used in this study.
  • Table S19. siRNA sequences for all genes that passed the secondary screen.

Format: Microsoft Excel

Size: 8.66 MB (compressed); 34.2 MB (decompressed)

Excel file is packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
  3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; www.winzip.com) or StuffIt Expander (Windows and Mac; www.stuffit.com).

Excel files can be opened and viewed using Microsoft Excel, the spreadsheet module of the freely downloadable Open Office suite, or the freely downloadable Excel Viewer available from Microsoft.

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Supplementary Materials (Cytoscape Files)

  • Cytoscape File 1. AGGF1. Cytoscape file for Figure 5B.
  • Cytoscape File 2. MSDATA. Cytoscape file for Figure 4.
  • Cytoscape File 3. WNT+DLDSEC. Cytoscape file for Figure 5A.

Format: Cytoscape (.cys)

Size: 246 KB (compressed); 81 KB (decompressed for AGGF1.cys); 102 KB (decompressed for MSDATA.cys); 76 (decompressed for WNT+DLDSEC.cys)

These three files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
  3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; www.winzip.com) or StuffIt Expander (Windows and Mac; www.stuffit.com).

Cytoscape files can be opened and viewed using the Cytoscape program, which can be freely downloaded from http://www.cytoscape.org/.

[Download Cytoscape Files]


*These authors contributed equally to this work.

†To whom correspondence should be addressed. E-mail: william_arthur{at}merck.com (W.T.A.) and rtmoon{at}u.washington.edu (R.T.M.)

Citation: M. B. Major, B. S. Roberts, J. D. Berndt, S. Marine, J. Anastas, N. Chung, M. Ferrer, X. Yi, C. L. Stoick-Cooper, P. D. von Haller, L. Kategaya, A. Chien, S. Angers, M. MacCoss, M. A. Cleary, W. T. Arthur, R. T. Moon, New regulators of Wnt/β-catenin signaling revealed by integrative molecular screening. Sci. Signal. 1, ra12 (2008).

© 2008 American Association for the Advancement of Science


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Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882