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Sci. Signal., 8 December 2009
[DOI: 10.1126/scisignal.2000610]

Supplementary Materials for:

Delivery of MicroRNA-126 by Apoptotic Bodies Induces CXCL12-Dependent Vascular Protection

Alma Zernecke,* Kiril Bidzhekov, Heidi Noels, Erdenechimeg Shagdarsuren, Lin Gan, Bernd Denecke, Mihail Hristov, Thomas Köppel, Maliheh Nazari Jahantigh, Esther Lutgens, Shusheng Wang, Eric N. Olson, Andreas Schober, Christian Weber*

*To whom correspondence should be addressed. E-mail: cweber{at}ukaachen.de (C.W.) and alma.zernecke{at}virchow.uni-wuerzburg.de. (A.Z.)

This PDF file includes:

  • Fig. S1. Identification of the targets of miR-126.
  • Fig. S2. Repression of luciferase reporter activity by RGS16, CXCL12, and mutated controls.
  • Fig. S3. Comparative analysis of the abundance of RGS3 and RGS16.
  • Fig. S4. Increased abundance of RGS16 in transfected HUVECs.
  • Fig. S5. CXCL12-mediated phosphorylation of ERK1/2 in HUVECs.
  • Fig. S6. Generation of apoptotic bodies by apoptotic HUVECs.
  • Fig. S7. Apoptotic bodies do not mobilize monocytes or neutrophils to peripheral blood.
  • Fig. S8. Sparse colocalization of Sca-1 with macrophages in atherosclerotic lesions of mice treated with apoptotic bodies.
  • Fig. S9. Apoptotic bodies isolated from human plaques and from mouse blood contain miR-126.
  • Fig. S10. Apoptotic bodies specifically inhibit apoptosis and induce the proliferation of HUVECs.
  • Table S1. The expression of miRNAs in SMCs.

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Citation: A. Zernecke, K. Bidzhekov, H. Noels, E. Shagdarsuren, L Gan, B. Denecke, M. Hristov, T. Köppel, M. N. Jahantigh, E. Lutgens, S. Wang, E. N. Olson, A. Schober, C. Weber, Delivery of MicroRNA-126 by Apoptotic Bodies Induces CXCL12-Dependent Vascular Protection. Sci. Signal. 2, ra81(2009).

© 2009 American Association for the Advancement of Science


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